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Quantitative analysis of dopaminergic synaptic function in human brain.

人脑多巴胺能突触功能的定量分析。

基本信息

批准号：
09470198
负责人：
YONEKURA Yoshiharu
金额：
$ 8.38万
依托单位：
Fukui Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470198/
关键词：
dopamine synaptic function functional neuroimaging PET SPECT

项目摘要

The aim of this study was to establish the methods for the quantitative analysis of dopaminergic synaptic function in human brain, basic an clinical sdudies were performed with various approach, including the kinetc analysis of SPECT imaging and basic experiments using fresh brain slices of rats. Serial dynamic SPECT imaging of I-123 iodobenzofuran (IBF) with intermittent arterial blood sampling permits to obtain binding potential of dopamine D2 receptors. In addition to the compartment analysis and simple target to nontarget ratio approach, we have developed a new graphical method which provides more reliable parameters on receptor binding. Dopamine transporter imaging was performed with I-123 fluoropropyl analogue of beta-CIT (FPCIT). FPCIT has an advantage of faster kinetics than beta-CIT, and test anr re-test brain SPECT scans demonstrated an excellent reproducibility of measuring the distribution volume in the striatum, suggesting that this tracer is promising for the clinical use. We also developed a system called dynamic positron autoradiography technique (dPAT) for serial imaging of fresh brain slices of rats with positron labeled radioligands. This new method permits the assessment of regional changes of radioactivity for several hours, and can be used to monitor glucose metabolism and receptor binding with various interventions, such as hypoxia and drug effects. It will play an important role in understanding the kinetic behaviour of radioligands.

本研究旨在建立人脑多巴胺能突触功能的定量分析方法，采用SPECT显像的动力学分析和大鼠新鲜脑片的基础实验等方法进行了基础和临床研究。连续动态SPECT成像的I-123碘苯并呋喃（IBF）与间歇性动脉血液采样允许获得多巴胺D2受体的结合潜力。除了房室分析和简单的目标与非目标比率的方法，我们已经开发了一种新的图形方法，提供更可靠的参数受体结合。用β-CIT的I-123氟丙基类似物（FPCIT）进行多巴胺转运蛋白成像。FPCIT具有比β-CIT更快的动力学优势，并且测试和再测试脑SPECT扫描显示测量纹状体分布体积的良好再现性，表明该示踪剂具有临床应用前景。我们还开发了一种称为动态正电子放射自显影技术（dPAT）的系统，用于正电子标记放射性配体的大鼠新鲜脑切片的连续成像。这种新方法允许评估几个小时的放射性区域变化，并可用于监测葡萄糖代谢和受体结合与各种干预措施，如缺氧和药物作用。它将在理解放射性配体的动力学行为中发挥重要作用。