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Genetic population structure of a long-finned squid photolologo edulis(Cephalopoda : Loliginidae)

长鳍鱿鱼的遗传种群结构（头足类：Loliginidae）

基本信息

批准号：
09660206
负责人：
NATSUKARI Yutaka
金额：
$ 1.28万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Genetic population structure of the commercially fished loliginid squid Photololigo edulis was examined by collecting three samples (294 individuals) of two seasonal populations from the East China Sea, and subjecting individuals to electrophoretic analysis.Twenty-three enzymes were tested, and 10 enzymes were screened for polymorphism. Clearly resolved enzyme phenotypes were obtained at seven (malate dehydrogenase, MDH-1ィイD1*ィエD1, MDH-2ィイD1*ィエD1, MDH-3ィイD1*ィエD1, E.C. 1.1.1.37 ; phosphogluconate dehydrogenase, PGDHィイD1*ィエD1, E.C.1.1.1.44 ; aspartate aminotransferase, AAT-2ィイD1*ィエD1, E.C.2.6.1.1; adenylate kinase, AK-2ィイD1*ィエD1, E.C.2.7.4.3 ; phosphoglucomutase, PGMィイD1*ィエD1, E.C.5.4.2.2). Routine scoring at the 7 loci revealed low levels of genetic variability, with the proportion of polymorphic loci, P=0-0.1, mean heterozygosities per locus=0.28-2.19 x10ィイD1-2ィエD1.Analyses of allele frequencies at seven polymorphic loci provided no evidence of genetic separation, and the frequency distribution of genotypic classes almost fitted Hardy-Weinberg expectations for randomly mating population. Nei's mean genetic distances between samples ranged from 0.491-2.522(x10ィイD1-4ィエD1), respectively, supporting the opinion that genetic population structure of the squid is homogeneous.

通过采集东海2个季节性鱿鱼种群的3份样本（294只），对商业捕捞鱿鱼的遗传群体结构进行了电泳分析。共检测23种酶，筛选出10种酶多态性。明确解决酶表型获得7点(苹果酸脱氢酶,MDH-1ィイD1 *ィエD1, MDH-2ィイD1 *ィエD1, MDH-3ィイD1 *ィエD1,提到过1.1.1.37;phosphogluconate脱氢酶,PGDHィイD1 *ィエD1, E.C.1.1.1.44;天冬氨酸转氨酶,AAT-2ィイD1 *ィエD1, E.C.2.6.1.1;腺苷酸激酶,AK-2ィイD1 *ィエD1, E.C.2.7.4.3; phosphoglucomutase, PGMィイD1 *ィエD1, E.C.5.4.2.2)。7个位点的常规评分显示遗传变异水平较低，多态性位点占比P=0 ~ 0.1，每个位点的平均杂合度=0.28 ~ 2.19 × 10 μ l D1 ~ 2 μ l D1。7个多态性位点的等位基因频率分析没有发现遗传分离的证据，基因型类别的频率分布几乎符合Hardy-Weinberg对随机交配群体的期望。Nei的平均遗传距离为0.491 ~ 2.522(x10 μ l D1 ~ 1 μ l D1)，支持鱿鱼遗传群体结构具有同质性的观点。