Molecular biological studies on classification and phylogeny of intestinal parasitic protozoa Blastocytis

肠道寄生原虫胚泡分类和系统发育的分子生物学研究

• 批准号: 09670257
• 负责人: YOSHIKAWA Hisao
• 金额: $ 2.05万
• 依托单位: Nara Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670257/
• 关键词: Blastocystis; protozoa; parasite; classification; genotype; PCR; DNA; DNA

The genotype Blastocystis hominis is highly polymorphic. Therefore, a genetic marker would be a powerful tool for the identification or classification of B.hominis subtypes and could be used as a means to resolve the transmission route or origin of the parasite. To this end, approximately 100 Blastocystis isolates were collected from humans and animals, and these organisms were subjected to genotype analysis based on diagnostic PCR primers. A total of 9 PCR primers were developed from randomly amplified polymorphic DNA products of Blastocystis isolates recovered from humans or animals. Based on PCR amplification using diagnostic PCR primers, approximately 50 isolates recovered from humans were classified into four different subtypes. In addition, these PCR primers were examined with approximately 50 isolates recovered from animals and 3 isolates from chickens, 3 isolates from quails, and 2 isolates from monkeys were amplified with the diagnostic PCR primers. Therefore, these amplified strains were identified as zoonotic B.hominis strains.

人芽囊原虫的基因型具有高度多态性。因此，遗传标记将是鉴定或分类人型B杆菌亚型的有力工具，并可用作解析寄生虫传播途径或起源的手段。为此，从人类和动物中收集了约100株芽囊原虫分离株，并基于诊断PCR引物对这些微生物进行基因型分析。从人或动物体内回收的芽囊原虫分离株随机扩增多态性DNA产物开发了总共9条PCR引物。基于使用诊断PCR引物的PCR扩增，将从人类回收的约50株分离株分为四种不同的亚型。此外，这些PCR引物与约50个从动物中回收的分离株进行了检查，并使用诊断PCR引物对3个从鸡中分离的分离株、3个从鹌鹑中分离的分离株和2个从猴中分离的分离株进行了扩增。因此，这些扩增菌株被鉴定为人畜共患B.hominis菌株。

项目成果

Hisao Yoshikawa: "Genomic analysis of Blastocystis hominis strains isolated from two long-term health care facilities"Journal of Clinical Microbiology. 38,4(in press). (2000)

Hisao Yoshikawa：“从两个长期医疗机构分离出的人芽囊菌菌株的基因组分析”临床微生物学杂志。

Hisao Yoshikawa: "Genomic polymorphism among Blastocystis hominis strains and development of subtype-specific diagnostic primers"Molecular and Cellular Probe. 12. 153-159 (1998)

Hisao Yoshikawa：“人芽囊藻菌株的基因组多态性和亚型特异性诊断引物的开发”分子和细胞探针。

Hisao Yoshikawa: "Genomic analysis of Blastocystis hominis strains isolated from two long-term health care facilities"Journal of Clinical Microbiology. 38 (in press). (2000)

Hisao Yoshikawa：“从两个长期医疗机构分离出的人芽囊菌菌株的基因组分析”临床微生物学杂志。

H.Yoshikawa,I.Nagano,Z.Wu,他3名: "Genomic polymorphism among Blastocystis hominis strains and development of subtype-specific diagnostic primers" Molecular and Cellular Probes. 12. 153-159 (1998)

H. Yoshikawa、I. Nagano、Z. Wu 和其他 3 人：“人芽胞藻菌株的基因组多态性和亚型特异性诊断引物的开发”《分子和细胞探针》12. 153-159 (1998)。

Hisao Yoshikawa: "Ultrastructural localization of basic proteins of Blastocystis hominis"Protoplasma. 200. 31-34 (1997)

Hisao Yoshikawa：“人芽囊藻碱性蛋白的超微结构定位”原生质体。