Identification of zoonotic isolates of Blastocystis by using polymorphic genomic DNA

利用多态性基因组 DNA 鉴定人畜共患芽囊菌分离株

• 批准号: 13670245
• 负责人: YOSHIKAWA Hisao
• 金额: $ 2.24万
• 依托单位: Nara Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670245/
• 关键词: Blastocystis; Genome; Polymorphism; Zoonotic; PCR; Primer; Genotype; Identification; 原生動物; 寄生虫; 分子生物学; 遺伝子プライマー

Since Blastocystis organisms have been widely isolated from a variety animals and extensive genetic and karyotypic heterogeneity have been demonstrated among Blastocystis isolates from humans and animals, it is difficult to clarify whether isolated organisms from animals are identical to or different from human B.hominis. In this study, therefore, we developed several sequenced-tagged site (STS) primers from random amplified polymorphic DNA analysis of known strains of B.hominis. Since the STS primers only amplified the distinct subtypes which correspond to the phylogenetically different clades inferred from the small subunit ribosomal DNA sequences, the STS primers can be used as a tool to type genotypes among polymorphic Blastocystis populations. When various isolates from mammals and birds have examined with PCR amplification by using the STS primers, most of the isolates were amplified with distinct primer, suggesting a zoonotic genotypes of animal isolates. These results indicated that Blastocystis organisms isolated from animals are potential sources of human infections.

由于芽囊原虫生物体已被广泛地从各种动物中分离出来，并且在人类和动物的芽囊原虫分离株中已证明了广泛的遗传和核型异质性，因此很难澄清从动物中分离的生物体是否与人类芽囊原虫相同或不同。因此，在这项研究中，我们开发了几个序列标记位点（STS）的引物从随机扩增多态性DNA分析已知菌株的人芽抱杆菌。由于STS引物仅扩增与从小亚基核糖体DNA序列推断的系统发育不同分支相对应的不同亚型，因此STS引物可用作多态性芽囊原虫种群中基因型分型的工具。用STS引物对来自哺乳动物和鸟类的分离株进行PCR扩增，大多数分离株的扩增产物具有明显的特异性，表明动物分离株具有人畜共患病的基因型。这些结果表明，从动物中分离的芽囊原虫是人类感染的潜在来源。

项目成果

Arisue Nobuko: "Sequence heterogeneity of the small subunit ribosomal RNA genes among Blastocystis isolates."Parasitology. 126. 1-9 (2003)

Arisue Nobuko：“芽囊菌分离株中小亚基核糖体 RNA 基因的序列异质性。”寄生虫学。

Yoshikawa Hisao: "Polymerase chain reaction-based genotype classification of human Blastocystis hominis populations isolated from different countries."Parasitology Research. 92. 22-29 (2004)

Yoshikawa Hisao：“基于聚合酶链反应的从不同国家分离的人类人芽囊菌种群的基因型分类。”寄生虫学研究。

Hisao Yoshikawa, Minako Nakajima, Keiko Morimoto, Yasuko Yamanouchi, Eu Hian Yap, Mulkt Singh.: "Freeze-fracture and cytochemical studies on the in vitro cyst form of reptilian Blastocystis pythoni."Journal of Eukaryotic Microbiology. 50. 70-75 (2003)

Hisao Yoshikawa、Minako Nakajima、Keiko Morimoto、Yasuko Yamanouchi、Eu Hian Yap、Mulkt Singh.：“爬行动物蟒蛇芽囊菌体外包囊形式的冷冻断裂和细胞化学研究。”真核微生物学杂志。

Nobuko Arisue, Tetsuo Hashimoto, Hisao Yoshikawa, Yoshihiro Nakamura, Gen Nakamura, Fuminori Nakamura, Taka-Aki Yano, Masami Hasegawa: "Phylogenetic position of Blastocystis hominis and stramenopiles inferred from multiple molecular sequence data."Journal

Nobuko Arisue、Tetsuo Hashimoto、Hisao Yoshikawa、Yoshihiro Nakamura、Gen Nakamura、Fuminori Nakamura、Taka-Aki Yano、Masami Hasekawa：“从多个分子序列数据推断人芽囊原虫和原生藻菌的系统发育位置。”杂志

Niichiro Abe, Zhiliang Wu, Hisao Yoshikawa: "Molecular characterization of Blastocystis isolates from primates."Veterinary Parasitology. 113. 321-325 (2003)

Niichiro Abe、Zhiliang Wu、Hisao Yoshikawa：“从灵长类动物中分离出的芽囊菌的分子特征。”兽医寄生虫学。