Epidemiology of tsutsugamushi disease in Kagoshima Prefecture

鹿儿岛县恙虫病流行病学

• 批准号: 09670269
• 负责人: NODA Shinichi
• 金额: $ 1.98万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670269/
• 关键词: tsutsugamushi disease; Orientia tsutsugamushi; Kagoshima; Leptotrombidium scutellare; PCR; 鹿児島県; 植生; 血清型

The collection of Leptotrombidium scutellare was carried out on grassland, natural woods (chinquapin and oak) and Japanese cedar woods. Many L. scutellare larvae were collected on natural woods and Japanese cedar woods as well as on grassland. The natural woods and Japanese cedar woods were suitable habitats for L. scutellare in Kagoshima Prefecture.The distribution of L. scutellare larva was examined along roads of mountainside. L. scutellare larvae were collected using the black cloth method at intervals of one kilometer, and larvae were found out at 41 points out of 52 points (78.8%). L. scutellare seemed to be widely distributed in Kagoshima Prefecture.Apodemus speciosus was captured and the antibody against Orientia tsutssugamushi was examined. Ten out of 18 (55.6%) had the antibody against O. tsutsugamushi. Wild rodents in Kagoshima seemed to have the antibody against O. tsutsugamushi at high rate.L. scutellare larvae were collected using black cloth method at 5 points of garden and 15 points of woods where patients were suspected to have contact with the tsutsugamushi disease at these areas. The detection of O. tsutsugamushi DNA from collected L. scutellare was carried out by Two-step PCR. Rickettsial DNA was detected from L. scutellare collected at one point of garden and 3 points of woods.The analysis of rickettsial types by endonuclease fragment length polymorphism of PCR products was carried out, and the rickettsial type was identified as Karp, Kawasaki or Kuroki types.

在草地、天然林（栗树和橡树）和日本雪松林中进行了小盾纤恙螨的收集。许多湖在天然林和日本雪松林以及草地上收集了小盾蚧幼虫。天然林和柳杉林是其适宜的生境; scutellare的分布。沿山坡沿着检查小盾蚧幼虫。L.用黑布法每隔1公里采集一次小盾幼虫，在52个点中的41个点（78.8%）发现幼虫。L.在鹿儿岛县广泛分布着恙虫病东方体，并捕获了美丽姬鼠，进行了恙虫病东方体抗体检测。18例中10例（55.6%）有抗O.恙虫病。鹿儿岛的野生啮齿动物似乎有抗O.恙虫病的高发病率。用黑布法在花园5处和树林15处疑似恙虫病接触点采集恙虫幼虫。对O.从采集的恙虫病标本中提取恙虫病DNA。采用两步PCR方法对黄芩的DNA进行扩增。从L.采用PCR扩增产物的酶切片段长度多态性分析方法，对采集的1个园林点和3个树林点的1株黄芩进行立克次体分型，将立克次体分型为Karp型、川崎型和Kuroki型。