Immune mechanisms in parasite-snail interactions

研究了副棘孔虫幼虫的排泄-分泌（E-S）产物对光斑生物phalaria glabrata造血器官和血细胞运动的影响。将卵泡悬浮于培养基中，孵育出幼虫，收集E-S产物。用E-S产品注射m系光斑田螺，并在组织学切片上确定HO的大小。注射孢子囊E-S产物的钉螺HO的大小明显大于注射培养基的钉螺HO的大小。采用超滤膜、聚丙烯酰胺凝胶电泳和电泳洗脱对孢子囊E-S产物进行分离。对孢子囊E-S产物的分离检测表明，E-S产物的特定成分负责刺激ho活性。整个（未分馏）和分馏的E-S产品在趋化室中进行测试。采用超滤膜对孢子囊的E-S产物进行分离。孢子囊E-S产物显著抑制m系光棘球蚴血细胞的随机运动。

Effects of excretory-secretory (E-S) products of Echinostoma paraensei larvae on hematopoietic organ and hemocyte motility of Biomphalaria glabrata were studied.Eggs of E. paraensei were suspended in culture medium, and hatched larvae were cultured to collect E-S products. M-line B. glabrata snails were injected with E-S products, and the size of the HO was ascertained in histological sections. The size of HO in snails injected with E-S products from sporocysts was significantly larger than that in snails injected with culture medium. E-S products of sporocysts were fractionated using ultrafiltration membranes, polyacrylamide gel electrophoresis, and electrophoretic elution. Examination of fractionated E-S products of sporocysts revealed that specific components of E-S products were responsible for HO-stimulating activity.Both whole (unfractionated) and fractionated E-S products were tested in chemotaxis chambers. E-S products of sporocysts were fractionated using ultrafiltration membranes. E-S products of sporocysts significantly inhibited the random motility of hemocytes from M-line B. glabrata.