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Development of immunoscintigraphy using radiolabeled murine anti-smooth muscle myosin monoclonal antibody for diagnostic imaging of acute bowel infarction-application of immunoscintigraphy for smooth muscle affecting disease-

使用放射性标记鼠抗平滑肌肌球蛋白单克隆抗体开发免疫闪烁扫描法，用于急性肠梗塞的诊断成像-免疫闪烁扫描法在平滑肌影响疾病中的应用-

基本信息

批准号：
09670695
负责人：
IWASAKI Tsutomu
金额：
$ 2.24万
依托单位：
Gunma University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Aortic dissection and acute bowel infarction are fatal diseases characterized by the extensive damage of smooth muscle cells. Prompt and accurate diagnosis-regarding the sit and extent of the lesion is necessary for the management of these catastrophes. The purpose of this research was to examine the feasibility of radioimmunoscintigraphy using ^<99m> Tc-anti-smooth muscle myosin antibody (SM-MAb) as a noninvasive diagnostic tool for aortic dissection and acute bowel infarction. The accumulation of ^<99m>Tc-anti-SM-MAb was studied and scintigraphic imaging with ^<99m>Tc-anti-SM-MAb was performed in the rats with experimental aortic dissection or acute bowel infarction. Rats with aortic dissection were further investigated 1 or 2 weeks after dissecting injury. The radioactivity of ^<99m>Tc-anti-SM-MAb in the dissected aorta or infarcted intestine showed significant increase compared to either normal portion or blood at 6-h post-injection of the radiotracer (the ratio of percent injected dose per gram (%ID/g) for the lesion vs. the normal portion ; 4.35 * 1.47 (aorta), 3.60*1.98 (intestine)). The scintigraphic imaging with ^<99m>Tc-anti-SM-MAb allowed distinct visualization of the dissected aorta or infarcted intestine with specific accumulation of antibody at 6-h post-injection of the radiotracer. Moreover, selective accumulation of the tracer to the dissected aorta was still observed allowing clear visualization of the dissected lesion by ^<99m>Tc-anti-SM-MAb immunoscintigraphy even at 1-week after aortic injury. These results were reported at 13^<th> World Congress of Angiology and 45^<th> Annual Meeting of Japanese. College of Cardiology, and published in Journal of Cardiology (Vol.31, 1998).

主动脉夹层和急性肠梗死是以平滑肌细胞广泛损伤为特征的致死性疾病。及时和准确的诊断-关于病变的位置和范围是必要的管理这些灾难。本研究的目的是探讨放射免疫显像的可行性，使用抗<99m>平滑肌肌球蛋白抗体（SM-MAb）作为一种非侵入性的诊断工具，主动脉夹层和急性肠梗死。本文<99m>研究了<99m>实验性主动脉夹层和急性肠梗塞大鼠的抗-SM-MAb的蓄积和抗-SM-MAb显像。主动脉夹层大鼠在夹层损伤后1或2周进一步观察。在<99m>注射放射性示踪剂后6小时，与正常部分或血液相比，解剖的主动脉或梗塞肠中的14 Tc-抗SM-MAb的放射性显示出显著增加（病变与正常部分的每克注射剂量百分比（%ID/g）的比率; 4.35 * 1.47（主动脉），3.60*1.98（肠））。使用14 Tc-抗SM-MAb的放射性造影成像<99m>允许在注射放射性示踪剂后6小时清楚地显示夹层主动脉或具有抗体特异性积聚的梗塞肠。此外，即使在主动脉损伤后1周，仍然观察到示踪剂在夹层主动脉中的选择性积聚，从而允许通过^<99m>Tc-抗SM-MAb免疫闪烁成像清晰地观察夹层病变。这些结果在第13届<th>世界血管病学大会和第45届<th>日本年会上报告。发表于《心脏病学杂志》（1998年第31卷）。