Modulation of cardiac potassium channels in pathological conditions and developments

在这项研究中。我们研究了在不同类型的病理条件和发育变化过程中心脏钾通道的调节。并探讨了基因调控通道的可能作用。K通道mRNA的水平。观察培养心肌细胞和肥厚心肌细胞中通道蛋白、空间分布通道蛋白及离子电流水平。本研究主要发现：1)甲状腺激素对各心脏通道的影响不同。2)单致右心室肥厚时动作电位持续时间的延长最初是由钙电流升高引起的，后来是由瞬时外电流降低引起的。3)对心肌通道的影响与心肌肥厚的类型有关。不同的调制取决于。以上研究结果均已接受在后面列出的期刊上发表。本研究中使用的分子方法都是近十年来发展起来的。这些在方法学上的显著进步可能会导致对患病心脏通道行为的进一步理解。

In this study. we examined the modulation of cardiac potassium channels in various types of pathological conditions and during developmental changes. Possible role of gene regulation of channels was also examined. Levels of K channel mRNA.levels of channel protein, spatial distribution channel protein and ion currents were observed in cultured cardiac myocytes or hypertrophied hearts. Main findings obtained during this grant were :1) Thyroid hormone exhibit diffeerent effects on each cardiac channels.2) The prolongation of action potential duration in monocrotalin-induced right ventricular hypertrophy was caused initially by increase in calcium current and lately by decrease in transient outward current.3) Effects on cardiac channels were dependent on the types of cardiac hypertrophy. differently modulated depends.All of above findings have already accepted to publish in journals listed later.The molecular methods used in this study were all developed in these ten years. These remarkable progresses in methodology may lead to further understanding of channel behavior in diseased hearts.