Modulation of cardiac potassium channels in pathological conditions and developments

心脏钾通道在病理状况和发展中的调节

• 批准号: 09670710
• 负责人: KAMIYA Kaichiro
• 金额: $ 0.45万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670710/
• 关键词: thyroid hormone; action potential; potassium channels; gene; cardiac hypertrophy; ion channels; アミオダロン

In this study. we examined the modulation of cardiac potassium channels in various types of pathological conditions and during developmental changes. Possible role of gene regulation of channels was also examined. Levels of K channel mRNA.levels of channel protein, spatial distribution channel protein and ion currents were observed in cultured cardiac myocytes or hypertrophied hearts. Main findings obtained during this grant were :1) Thyroid hormone exhibit diffeerent effects on each cardiac channels.2) The prolongation of action potential duration in monocrotalin-induced right ventricular hypertrophy was caused initially by increase in calcium current and lately by decrease in transient outward current.3) Effects on cardiac channels were dependent on the types of cardiac hypertrophy. differently modulated depends.All of above findings have already accepted to publish in journals listed later.The molecular methods used in this study were all developed in these ten years. These remarkable progresses in methodology may lead to further understanding of channel behavior in diseased hearts.

本研究我们检测了在各种病理条件下和发育变化期间心脏钾通道的调节。还研究了通道基因调控的可能作用。在培养的心肌细胞和肥大的心脏上观察K通道mRNA水平、通道蛋白、空间分布通道蛋白和离子电流的变化。结果表明：1）甲状腺激素对心肌各通道具有不同的作用; 2）野百合碱诱导的右心室肥厚，动作电位时程的延长主要是由钙电流增加引起，随后是瞬时外向电流减少引起; 3）对心肌通道的作用与心肌肥厚的类型有关。不同的调制取决于。所有上述研究结果已经接受发表在期刊后列出。本研究中使用的分子方法都是在这十年发展起来的。这些方法学上的显着进展可能会进一步了解患病心脏的通道行为。

项目成果

GUO Weinong, KAMIYA Kaichiro, YASUI Kenji, KODAMA Itsuo, TOYAMA Junji: "alpha_1-Adrenoceptor agonists and IGF-1, myocardial hypertrophic factors, regulate the Kv1.5 K^- channel expression differentially in cultured newborn rat ventricular cells." Pflugers

GUO Weinong、KAMIYA Kaichiro、YASUI Kenji、KODAMA Ituo、TOYAMA Junji：“α_1-肾上腺素受体激动剂和 IGF-1、心肌肥大因子，在培养的新生大鼠心室细胞中差异调节 Kv1.5 K^- 通道表达。”

HISHIYAMA A., KAMBE F., KAMIYA K., et al.: "Effects of thyroid status on expression of voltage-gated potassium channels." Cardiovascular Research. 40. 343-351 (1998)

HISHIYAMA A.、KAMBE F.、KAMIYA K. 等人：“甲状腺状态对电压门控钾通道表达的影响。”

NISHIYAMA Atsushi,KAMBE Fukushi,KAMIYA Kaichiro,SEO Hisao,TOYAMA Junji:"Effects of thyroid hormone on the expression of Kv4.2 and Kv 4.3 potassium channel genes in the rat heart." Environmental Medicine. 41.2. 117-119 (1997)

NISHIYAMA Atsushi、KAMBE Fukushi、KAMIYA Kaichiro、SEO Hisao、TOYAMA Junji：“甲状腺激素对大鼠心脏 Kv4.2 和 Kv 4.3 钾通道基因表达的影响。”

神谷香一郎,外山淳治: "不整脈の電気生理学的機序." 「抗不整脈薬のすべて」Part2基礎編. 49-93 (1997)

Koichiro Kamiya，Junji Toyama：“心律失常的电生理机制”。“关于抗心律失常药物”第 2 部分基础版（1997 年）。

Kamiya K.,YOSHIDA S.,HOJO M.et al.: "Acute effects of amiodarone on E-4031-sensitive (I_<Kr>) and 293B-sensitive (I_<Ks>) components of delaved rectifier potassium channels in rabbit ventricular myocytes." Environmental Medicine. 42. 128-130 (1998)

Kamiya K.、YOSHIDA S.、HOJO M.等人：“胺碘酮对兔失活整流钾通道的 E-4031 敏感 (I_<Kr>) 和 293B 敏感 (I_<Ks>) 成分的急性影响