Modulation of cardiac potassium channels in pathological conditions and developments

在这项研究中。我们研究了心脏钾通道在不同类型的病理条件下和发育变化过程中的调节。此外，还研究了基因调控通道的可能作用。在培养的心肌细胞和肥厚的心脏中观察到钾通道mRNAs水平、通道蛋白水平、空间分布通道蛋白和离子电流。本研究的主要发现是：1)甲状腺激素对不同的心脏通道表现出不同的作用。2)野百合素引起的右室肥厚的动作电位时程延长最初是由于钙电流的增加，后来是由于瞬时外向电流的减少。3)对心脏通道的影响取决于心肌肥厚的类型。所有上述发现都已被接受发表在后面列出的期刊上。这项研究中使用的分子方法都是在这十年中发展起来的。方法学上的这些显著进展可能会导致对疾病心脏经络行为的进一步了解。

In this study. we examined the modulation of cardiac potassium channels in various types of pathological conditions and during developmental changes. Possible role of gene regulation of channels was also examined. Levels of K channel mRNA.levels of channel protein, spatial distribution channel protein and ion currents were observed in cultured cardiac myocytes or hypertrophied hearts. Main findings obtained during this grant were :1) Thyroid hormone exhibit diffeerent effects on each cardiac channels.2) The prolongation of action potential duration in monocrotalin-induced right ventricular hypertrophy was caused initially by increase in calcium current and lately by decrease in transient outward current.3) Effects on cardiac channels were dependent on the types of cardiac hypertrophy. differently modulated depends.All of above findings have already accepted to publish in journals listed later.The molecular methods used in this study were all developed in these ten years. These remarkable progresses in methodology may lead to further understanding of channel behavior in diseased hearts.