Physiological intrahepatic cholestasis by breast milk

母乳引起的生理性肝内胆汁淤积

• 批准号: 09670808
• 负责人: MURA Tatsuo
• 金额: $ 1.86万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670808/
• 关键词: biliary obstruction; sinusoidal bile salt transport protein; basolateral plasma membrane; bile salt-dependent flow; osmolarity; multi variate regression of bile flow; breast milk; intracellular cyclic AMP level; 新生児; 放射性胆汁酸; ラット小腸ループ法; 胆汁酸消失率 /

Studies of bile flow physiology are hampered by the lack of techniques such as micropuncture, which might directly assess different components of bile flow. Indirect techniques such as linear regression analysis and measurement of canalicuar flow with markers, which are 14C-labelled bile acids, must be interpreted cautiously. In these studies, we have set out to examine the cause of the large range of effect that a single bile acid or in combination of breast milk can have on bile flow.The following aspects of the present study appear to be interpretable with some confidence. The variable effect of bile acid is not accompanied by changes in bile osmolarlity. Second, a good correlation exists between the effect of bile salts on flow and the mass of electrolyte secreted in conjugation with bile salts. Finally, when breast milk addition can not reduce the effect of bile salt on flow, an action previously demonstrated by bile salt-associated electrolyte secretion is not reduced.It seems reasonable to conclude that the variable effect of bile salt on bile flow is not due to generation of fluid of variable tonicity. The final conclusion is based on the fact that breast milk is substance which alters cellular electrolyte transport and has been used extensively in previous studies to do so. It raises intracellular cyclic AMP levels, which it is of the couple or electrogenic type. It is very unlikely that the effect secondary acting on the liver.Breast milk is not a physiological regulator, but the fact that variations in the control rat study were spontaneous and unrelated to the time from surgery suggests that there are physiological regulators. Furthermore, these results suggest that hormones in breast milk may be the regulators, especially somatostatin.

胆汁流动生理学的研究受到缺乏诸如显微穿刺等技术的阻碍，这些技术可以直接评估胆汁流动的不同成分。间接技术如线性回归分析和用标记物（14C标记的胆汁酸）测量导管流量，必须谨慎解释。在这些研究中，我们已经着手研究单一胆汁酸或与母乳结合对胆汁流量产生大范围影响的原因。胆汁酸的可变效应不伴有胆汁渗透压的变化。其次，胆汁盐对流量的影响与胆汁盐结合分泌的电解质质量之间存在良好的相关性。最后，当母乳添加不能降低胆盐对胆汁流量的影响时，先前通过胆盐相关电解质分泌证明的作用不会降低，似乎可以合理地得出结论，胆盐对胆汁流量的可变影响不是由于产生可变张力的液体。最后的结论是基于这样一个事实，即母乳是一种改变细胞电解质转运的物质，并且在以前的研究中已被广泛使用。它提高细胞内环AMP水平，它是夫妇或产电类型。母乳不是一种生理调节剂，但事实上，对照大鼠研究中的变化是自发的，与手术时间无关，这表明存在生理调节剂。此外，这些结果表明，母乳中的激素可能是调节剂，特别是生长抑素。

项目成果

Takafumi Nakamura, Tetsuo Mura, Kiyoko Saito, Tokuya Ohsawa, Hideo Akiyoshi and Kenzo Sato: "Adenovirus-Transferred HNF-38 Conserves Some Liver Functions in Primary Cultured Hepatocytes of Adult Rats"Biochemical And Biophysical Research Communications. 25

Takafumi Nakamura、Tetsuo Mura、Kiyoko Saito、Tokuya Ohsawa、Hideo Akiyoshi 和 Kenzo Sato：“腺病毒转移的 HNF-38 在成年大鼠原代培养肝细胞中保留部分肝功能”生化和生物物理研究通讯。

Takafumi Nakamura, Tetsuo Mura, 他: "Adenovirus-Transferred HNF-3γ conserves some Liver Functions in Primary Cultured Hepatocytes of Adult Rats"Biochemical and Biophysical Research Communications. 253. 352-357 (1998)

Takafumi Nakamura、Tetsuo Mura 等：“腺病毒转移的 HNF-3γ 在成年大鼠的原代培养肝细胞中保留了一些肝功能”生物化学和生物物理研究通讯。 253. 352-357 (1998)