The study on the autoantigens and their epitopes of autoimmune hemolytic anemia.

在自身免疫性溶血性贫血（AIHA）中，确定抗红细胞（RBC）自身抗体所针对的自身抗原的表位对于揭示免疫耐受失败的机制具有重要意义。本文分析了21例AIHA患者红细胞自身抗体的特异性。为了制备panel细胞，我们构建了编码4个Rh-cDNA的逆转录病毒基因，它们含有RhD、cE、ce和CE，并将它们导入KU 812 E细胞。流式细胞术证实除RhC外的所有Rh抗原均表达。用流式细胞术检测自身抗体对这些细胞的反应性。结果表明，21例中有10例（47.6%）对Rh多肽具有特异性。这10种自身抗体中有9种与RhC（c）E（e）多肽反应，3种与RhD多肽反应。提示RhC（c）E（e）多肽在AIHA的发病机制中起重要作用，其共同区存在自身抗体的抗原决定簇。

In autoimmune hemolytic anemia (AIHA), the determination of the epitopes of autoantigens directed with anti-red blood cells (RBC) autoantibodies is very important for revealing the mechanism in the failure of immunotolerance. We planed analysing the specificities of autoantibodies eluted from RBC of 21 AIHA patients. For the purpose of preparing panel cells, we constructed retroviral gene coding 4 Rh-cDNAs, which contain RhD, cE, ce, and CE, and introduced them into KU812E cells. All the Rh antigens other than RhC were confirmed to be expressed by flow cytometory. The reactivities of autoantihodies against these panel cells were examined by flow cytometory. The results showed specificities against the Rh polypeptides in 10 of 21 cases (47.6%). Nine of these 10 autoantibodies reacted with the RhC(c)E(e) polypeputide and three did with the RhD polypeptide. These data suggested that the RhC(c)E(e) polypeptide play an important role in pathogenesis of AIHA and that the epitopes directed with autoantibodies exist on the common region of the Rh polypeptides.