The role of synovial tissues to induce spontaneous remission for the inflammation by calcium pyrophosphote dihydrate crystals.

(材料和方法)用放射性同位素(Ca^&lt；45&gt；)制备二水焦磷酸钙(CPPD)晶体。将这些晶体中的5 mg注入大鼠的气囊(滑膜腔)。分别于注射后0、2、12、24、48、120、168h抽吸滑液，取出气囊中的滑膜组织。结果：1.各组滑液中CPPD含量分别为注射后2小时、6小时、12小时、12小时、24小时、48小时的总量的34.6%、20.7%、11.0%、1.9%、0.5%。滑膜组织溶解溶液中CPPD在注射后2小时为大鼠气囊注射总量的4.9%，6小时为65.6%，12小时为47.0%，24小时为28.7%，48小时为30%。滑膜组织学检查显示，6h后CPPD晶体附着于滑膜表面，未进入滑膜组织。24小时后，CPPD晶体和炎性细胞进入滑膜组织(滑膜内)。(结论)滑膜组织对晶体诱导的炎症有一定的自发缓解作用。

(materials and methods)Calcium pyrophosphate dihydrate (CPPD) crystals were prepared using radioisotope (Ca^<45>). Five mg of these crystal were injected into the rat air pouch (synovial space). Synovial fluid was aspirated and synovial tissues were removed from air pouch 0, 2, 12, 24, 48, 120, 168 hours after crystal injection. After centrifuging the synovial fluid and dissolving the synovial tissues of each group, CPPD crystal in supernates, sediments and dissolved solution of the synovial tissues were measured using liquid scintillation counter.(result)1. CPPD in the sediments were 3 4.6% of total amounts injected into rat air pouch at 2 hours after injection, 20.7% at 6 hours, 11.0% at 12 hours , 1.9% at 24 hours, 0.5% at 48 hours.2. CPPD in the dissolved solution of the synovial tissues were 4.9% of total amounts injected into rat air pouch at 2 hours after injection, 65.6% at 6 hours, 47.0% at 12 hours , 28.7% at 24 hours, 30% at 48 hours.3. Histological examination of the synovial tissue revealed that CPPD crystals were attached to the surface of synovium and did not taken into the synovial tissues at 6 hours. CPPD crystals and inflammatory cells were taken into the synovial tissues (inside the synovial membrane) at 24 hours.(conclusion) Synovial tissues have some role to induce spontaneous remission for crystal induced inflammation.