The role of synovial tissues to induce spontaneous remission for the inflammation by calcium pyrophosphote dihydrate crystals.

滑膜组织通过二水焦磷酸钙晶体诱导炎症自发缓解的作用。

• 批准号: 09671465
• 负责人: WATANABE Wataru
• 金额: $ 0.58万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671465/
• 关键词: calcium pyrophosphate dihydrate; crystal induced inflammation; synovium; spontaneous remission; radioisotope; air pouch; 自然緩解; air pouch

(materials and methods)Calcium pyrophosphate dihydrate (CPPD) crystals were prepared using radioisotope (Ca^<45>). Five mg of these crystal were injected into the rat air pouch (synovial space). Synovial fluid was aspirated and synovial tissues were removed from air pouch 0, 2, 12, 24, 48, 120, 168 hours after crystal injection. After centrifuging the synovial fluid and dissolving the synovial tissues of each group, CPPD crystal in supernates, sediments and dissolved solution of the synovial tissues were measured using liquid scintillation counter.(result)1. CPPD in the sediments were 3 4.6% of total amounts injected into rat air pouch at 2 hours after injection, 20.7% at 6 hours, 11.0% at 12 hours , 1.9% at 24 hours, 0.5% at 48 hours.2. CPPD in the dissolved solution of the synovial tissues were 4.9% of total amounts injected into rat air pouch at 2 hours after injection, 65.6% at 6 hours, 47.0% at 12 hours , 28.7% at 24 hours, 30% at 48 hours.3. Histological examination of the synovial tissue revealed that CPPD crystals were attached to the surface of synovium and did not taken into the synovial tissues at 6 hours. CPPD crystals and inflammatory cells were taken into the synovial tissues (inside the synovial membrane) at 24 hours.(conclusion) Synovial tissues have some role to induce spontaneous remission for crystal induced inflammation.

使用放射性同位素（Ca2+）制备焦磷酸钙二水合物（CPPD）晶体<45>。将5 mg这些晶体注射到大鼠气囊（滑膜间隙）中。分别于注射晶体后0、2、12、24、48、120、168 h抽取关节液，取出气囊内滑膜组织。取各组关节液，溶解滑膜组织，用液体闪烁计数器测定滑膜组织上清液、沉淀物和溶解液中CPPD晶体的含量。（结果）1.注射后2h、6h、12h、24h、48h沉积物中CPPD含量分别为注射总量的34.6%、20.7%、11.0%、1.9%、0.5%。注射后2h、6h、12h、24h、48h滑膜组织溶出液中CPPD分别占注射总量的4.9%、65.6%、47.0%、28.7%、30%。滑膜组织的组织学检查显示，CPPD晶体附着于滑膜表面，并且在6小时时没有进入滑膜组织。在24小时将CPPD晶体和炎性细胞带入滑膜组织（滑膜内）。（结论）滑膜组织对晶体诱导的炎症反应有一定的诱导缓解作用。