The action of IL-24 involving the exacerbation of pneumonia in RSV-infected mice exposed perinatally to a brominated flame retardant.

IL-24 的作用导致围产期接触溴化阻燃剂的 RSV 感染小鼠肺炎恶化。

• 批准号: 23590161
• 负责人: WATANABE Wataru
• 金额: $ 3.24万
• 依托单位: Kyushu University of Health and Welfare
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590161/
• 关键词: RSV; IL-24; 臭素化難燃剤; TBBPA; ウイルス; 免疫学; 有害化学物質; RSウイルス

To reveal effects of perinatal exposure to a brominated flame retardant TBBPA on the exacerbation of RSV-induced pneumonia, mechanism of action of IL-24 on RSV infection was investigated.By immune histopathological analysis, increase of the expression of IL-24 was confirmed in the lung tissues of RSV-infected mice exposed perinatally to TBBPA. The expression of IL-24 was also found in RA264.7 cells by real-time RT-PCR and western blotting in vitro. Although correlation between increase of IL-24 and decrease of a proinflammatory cytokine TNF-alpha was observed in the RSV-infected cells, direct interaction of them could not be revealed due to the failure of RNAi against IL-24 gene. Increase of IL-24 induced due to TBBPA-exposure might affect the production of proinflammatory cytokine from macrophages, should result in disorder of immune system responding to RSV infection.

为揭示溴化阻燃剂TBBPA对RSV肺炎的影响，本研究探讨了IL-24在RSV感染中的作用机制，并通过免疫组织病理学分析证实了TBBPA对RSV感染小鼠肺组织中IL-24表达的影响。RT-PCR和Western blotting检测结果显示，IL-24在RA264.7细胞中有表达。虽然在RSV感染的细胞中观察到IL-24的增加和促炎细胞因子TNF-α的减少之间的相关性，但由于针对IL-24基因的RNAi的失败，它们之间的直接相互作用不能被揭示。TBBPA暴露诱导的IL-24升高可能影响巨噬细胞产生促炎细胞因子，导致RSV感染后免疫系统应答紊乱。

项目成果

Effect of oral administration of probiotics from Mongolian dairy products on Th1 immune response in mice.

口服蒙古乳制品益生菌对小鼠 Th1 免疫反应的影响。

• 发表时间: 2013
• 期刊: Biosci. Biotechnol. Biochem.
• 作者: Takeda;S.;Kawahara;S.;Hidaka;M.;Yoshida;H.;Watanabe;W.;Takeshita;M.;Kikuchi;Y.;Bumbein;D.;Muguruma;M.;Kurokawa;M.
• 通讯作者: M.

Efficacy of Korean traditional medicines against influenza virus infection in mice and their modes of anti-influenza virus action

韩国传统药物对小鼠流感病毒感染的功效及其抗流感病毒作用方式

• 发表时间: 2011
• 期刊: J. Trad. Med.
• 作者: Shimizu;T.;Sawamura;R.;Kim;K-H.;Kim;K-H.;Lee;S-H.;Lee;Y-S.;Kim;T-G.;Kai;H.; Yoshida;H.;Watanabe;W.;Matsuno;K.;Shiraki;K.;Kurokawa;M.
• 通讯作者: M.

Perinatal exposure to an insecticide methamidophos affected the immune redponse to virus infection in the next generation of mice

围产期接触杀虫剂甲胺磷会影响下一代小鼠对病毒感染的免疫反应

• 发表时间: 2013
• 作者: Wataru Watanabe;Hiroki Yoshida;Akihiko Hirose;Masahiko Kurokawa
• 通讯作者: Masahiko Kurokawa

有機リン系殺虫剤メタミドホスの周産期暴露によるRSウイルス感染免疫への影響

围产期接触有机磷杀虫剂甲胺磷对呼吸道合胞病毒感染免疫力的影响

• 作者: 渡辺 渡;吉田裕樹，広瀬明彦，黒木奈緒，本郷聡子，柴田麻美;紺野克彦;黒川昌彦
• 通讯作者: 黒川昌彦

多層型カーボンナノチューブ曝露によるRSウイルス感染免疫への影響

接触多壁碳纳米管对呼吸道合胞病毒感染免疫力的影响

• 发表时间: 2014
• 作者: 橋口誠子;吉田裕樹;徳田健治;紺野克彦;明石敏;広瀬明彦;黒川昌彦;渡辺渡
• 通讯作者: 渡辺渡