Experimental study for elucidation of the onset mechanism in the phantom pain

阐明幻痛发病机制的实验研究

• 批准号: 09671572
• 负责人: TOTOKI Tadahide
• 金额: $ 1.86万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671572/
• 关键词: phantom pain; neuropathic pain; allodynia; neurotransmitters; neuropeptides; deafferentation pain; rats; immunohistochemistry

We attempted to develop an experimental animal model for peripheral neuropathic pain including the phantom pain. Under anesthesia, median, ulnar and raidiar nerves of one side of the rat (SD and hairless) were cut (group 1) or pull out (group 2). Sensitivity of the fore paw to mechanical stimulation was tested with von Frey hair for the next 5 months in two groups. The general behavior of each rat was noted during the entire test period.Following arm denervation, autotomy in 50% (group 1) and 20% (group 2) exploded. In the hairless rat, the ulcer of the skin was observed in 50% (both group). The test with mechanical stimulation in all groups did not produce a mechanical allodynia. Seven days following axotomy of the nerves, some large and medium sized cells in the dorsal root ganglia (C4〜Th1) displayed neuropeptide Y (NPY)-like immunoreactivity (IR), and marked increases in the numbers were observed for the next 5 months. The number of NPY-IR cells in the stellate ganglion (SG) increased in the group 2. A few strong staining cells for the nicotinamaide adenin dinucleotide phosphate ? Diaphorase (NADPH-d) were observed in the SG of the group 1 for the next 7 days. NADPH-d staining cells were not detected in the normal rat SG.We concluded that the study of the neuropathic pain including the phantom pain must be done using different species and families.

我们试图建立一种包括幻痛在内的周围神经病理性疼痛的实验动物模型。在麻醉状态下，切断SD无毛大鼠一侧正中神经、尺神经和桡神经（第1组）或拔出一侧正中神经、尺神经和桡神经（第2组）。在接下来的5个月里，在两组中用von Frey毛发测试前爪对机械刺激的敏感性。在整个实验过程中记录每只大鼠的一般行为，在手臂去神经后，50%（组1）和20%（组2）的自断发生爆炸。在无毛大鼠中，在50%（两组）中观察到皮肤溃疡。所有组中的机械刺激测试均未产生机械异常性疼痛。神经切断后7天，背根神经节（C4）内的一些大中型细胞（C4 + Th 1）呈神经肽Y（NPY）样免疫反应性（IR），并在随后的5个月内明显增多。第2组星状神经节（SG）内NPY阳性细胞数增多。有几个烟酰胺腺嘌呤二核苷酸磷酸的强染色细胞？在接下来的7天中，在组1的SG中观察到心肌黄酶（NADPH-d）。正常大鼠SG未见NADPH-d染色细胞。提示对神经病理性痛包括幻痛的研究应采用不同的种属和科属。