Secretion of the airway cilliated epithelial cell

气道纤毛上皮细胞的分泌

• 批准号: 09671770
• 负责人: MIWA Masato
• 金额: $ 2.11万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671770/
• 关键词: secretion; choride channel; ciliated epithelial cell; airway; patch clamp; allergy; macrolides; primary culture of epithelial cells

The Origin of airway secretion are thought to be composed of seromucous glands, goblet cells, transudation from capillaries secretions and cell debris from leucocytes. We demonstrated that the chloride conductance of tracheal ciliated epithelial cells excised from guinea pigs increases after administration of β-adrenergic, cholinergic and purinergic stimulants based on the patch clamp technique for the first time. We concluded that the pericilliary layers with low viscosity in which cilia beat is possibility formed partly by the ciliated epithelial cell itself.

呼吸道分泌物的来源被认为是由浆液腺、杯状细胞、毛细血管分泌物的转体和白细胞的细胞碎片组成的。我们首次基于膜片钳技术证明了从豚鼠身上切除的气管纤毛上皮细胞在给予β-肾上腺素能、胆碱能和嘌呤能兴奋剂后，氯离子电导增加。我们认为纤毛跳动的纤毛周层可能部分是由纤毛上皮细胞自身形成的。

项目成果

Komori-Matsunaga,M., Miwa,M., Iwata,S. et al.: "Effects of osmolarity changes in nebulizer solutions on human nasal muscosa"Otolaryngology Tokyo. 42. 181-185 (1999)

小森松永，M.，三轮，M.，岩田，S.

Hirano,M., Miwa,M., Iwata,S. et al: "Inhibitory effect of marolides on the electrolyte transport of the airway epithelium"Japanese Journal of Antibiotics. 50(suppl.A). 58-60 (1997)

平野，M.，美轮，M.，岩田，S。

Arimura,A., Miwa,M., Hasegawa,J. et al: "Characterization and localization of thromboxane AィイD22ィエD2 receptor in human and guinea pig nasal muscosa using radiolabelled (+) S-145"British Journal of Pharmacology. 124. 795-803 (1998)

Arimura, A.、Miwa, M.、Hasekawa, J. 等人：“使用放射性标记的 (+) S-145 对人类和豚鼠鼻粘膜中血栓素 A-D22-D2 受体进行表征和定位”《英国药理学杂志》。 124.795-803（1998）

三輪正人,他: "気道炎痕とマクロライド-到達点と今後の課題-" Japanese Journal of antibiotics. 52 in press. (1999)

Masato Miwa 等人：“呼吸道炎症疤痕和大环内酯类药物 - 目标和未来的挑战”，日本抗生素杂志 52 版（1999 年）。

平野光芳、他: "気道繊毛上皮細胞の電解質分泌に対するマクロライドの効果"Japanes Journal of Antibiotics. 51. 152-154 (1998)

Mitsuyoshi Hirano 等人：“大环内酯类药物对气道纤毛上皮细胞电解质分泌的影响”日本抗生素杂志 51. 152-154 (1998)。