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Dynamic analysis of physiological barrier function of nasal mucosal epithelia

鼻粘膜上皮生理屏障功能的动态分析

基本信息

批准号：
20592003
负责人：
MIWA Masato
金额：
$ 2.91万
依托单位：
Dokkyo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

项目摘要

The regulation of barrier function of nasal epithelium seems to be involved in the pathogenesis of allergic rhinitis. The measurement of transepidermal water loss has been proved to be an important non-invasive method for assessing the efficiency of the skin as a protective barrier. Although the nasal mucosal epithelium has such protective function as well as skin, the precise mechanism is still obscure. We examined the human nasal transepithelial water loss (TEWL) using our original adapter as well as the measurement of electrical potential differences (PD) of nasal mucosa. We observed that TEWL of the nose was decreased after topical application of hydrogen water rather than ascorbic acid.We could also examine PD of the posterior wall of the human pharyngeal mucosa. After application of HCl, PD was decreased compared with the basal state. After application of ZnCl2, PD was significantly increased compared with basal state.We could develop a new model to evaluate the effect of sheer s … More tress on the electrical barrier function of nasal mucosal epithelium. We have shown in this study that our original model might be very useful for analyzing the effect of shear stress on the airway epithelium. The data obtained from this model would contribute to understand the pathogenesis and therapy of the upper airway diseases with the rupture of the barrier function by pathological shear stresses.We have evaluated the short term effect of polycations and polyanions on the transepithelial electrical resistance (TER) of air-interfaced cultured nasal and tracheal epithelial cells excised from guinea pig and to determine the route (transcellualr or paracellular ) that cause the alteration of TER. The reduction in TER of the primary cultured nasal epithelial cells by polycations might be caused via the transcellualr route including ion channels as well as paracellular route like tight junction. Moreover, polyanion like heparin might recover the barrier function of airway mucosal epithelium after application of LPS and poly (I:C). Less

鼻黏膜屏障功能的调节可能参与了变应性鼻炎的发病机制。经皮水分损失的测量已被证明是评估皮肤作为保护屏障的效率的重要的非侵入性方法。虽然鼻粘膜上皮与皮肤一样具有这种保护作用，但其确切机制尚不清楚。我们使用我们的原始适配器检查了人鼻经上皮水分损失（TEWL）以及鼻粘膜电位差（PD）的测量。我们观察到局部应用氢水而不是抗坏血酸后鼻的TEWL降低。我们还可以检查人咽粘膜后壁的PD。应用盐酸后，PD与基础状态相比有所下降。应用ZnCl_2后，PD较基础状态显著增加，我们可以建立一个新的模型来评估剪切应力对PD的影响。 ...更多信息对鼻粘膜上皮电屏障功能的影响。在这项研究中，我们已经表明，我们的原始模型可能是非常有用的分析切应力对气道上皮细胞的影响。本实验观察了聚阳离子和聚阴离子对豚鼠气管上皮细胞跨上皮电阻（TER）的短期影响，并探讨了其作用途径。（transcellualr或paracellular），其引起TER的改变。聚阳离子降低原代培养鼻上皮细胞的TER可能是通过离子通道等跨细胞途径和紧密连接等细胞旁途径引起的。此外，聚阴离子肝素可恢复LPS和poly（I：C）作用后气道粘膜上皮的屏障功能。少