Molecular mechanism of membrane fusion in salivary exocytosis.
唾液胞吐作用中膜融合的分子机制。
基本信息
- 批准号:09671902
- 负责人:
- 金额:$ 1.92万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Parotid acinar cells contain VAMP-2 as a v-SNARE on secretory granule membranes, but do not have brain-type t-SNAREs, such as syntaxin-1 o SNAP-25. In order to examine whether or not syntaxin-4 and SNAP-23, plausible candidates of t-SNAREs in non-neuronal cells, can act as t-SNAREs in parotid acinar cells, we studied the protein-protein interaction among those proteins. Immunoblotting analysis showed that parotid acini contain both syntaxin-4 and SNAP-23. However, when VAMP-2 was immunoprecipitated from lysates of parotid acinar cells, syntaxin-4 and SNAP-23 were not coprecipitated with VAMP-2, although syntaxin-1 and SNAP-25 were clearly coprecipitated with VAMP-2 from brain lysates. *ersely, when syntaxin-4 was immunoprecipitated from parotid lysates, SNAP-23, Munc 18c, and NSF were coprecipitated, but VAMP-2 was again undetectable. When proteins in the crude secretory granule fraction were biotinylated and then immunoprecipitated with anti- VAMP-2, some proteins were coprecipitated along with VAMP-2. These results suggest that the interaction between VAMP-2 and syntaxin-4 or SNAP-23 was very weak or hindered by other proteins in parotid acinar cells.
腮腺腺泡细胞含有VAMP-2作为分泌颗粒膜上的v-SNARE,但不具有脑型t-SNARE,如突触融合蛋白-1或SNAP-25。为了研究是否syntaxin-4和SNAP-23,t-SNARE在非神经元细胞中的可能候选者,可以作为t-SNARE在腮腺腺泡细胞中,我们研究了这些蛋白质之间的蛋白质-蛋白质相互作用。免疫印迹分析显示腮腺腺泡含有syntaxin-4和SNAP-23。然而,当VAMP-2从腮腺腺泡细胞裂解物中免疫沉淀时,突触融合蛋白-4和SNAP-23不与VAMP-2共沉淀,尽管突触融合蛋白-1和SNAP-25与来自脑裂解物的VAMP-2明显共沉淀。相反,当从腮腺裂解物中免疫沉淀突触融合蛋白-4时,SNAP-23、Munc 18 c和NSF共沉淀,但VAMP-2再次检测不到。当粗分泌颗粒级分中的蛋白质被生物素化,然后用抗VAMP-2免疫沉淀时,一些蛋白质与VAMP-2一起沿着共沉淀。这些结果表明,VAMP-2和syntaxin-4或SNAP-23之间的相互作用是非常弱的,或被腮腺腺泡细胞中的其他蛋白质阻碍。
项目成果
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TAKUMA Taishin其他文献
TAKUMA Taishin的其他文献
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{{ truncateString('TAKUMA Taishin', 18)}}的其他基金
Identification of SNARE proteins for salivary exocytosis
唾液胞吐作用中 SNARE 蛋白的鉴定
- 批准号:
19592153 - 财政年份:2007
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Effect of siRNA for SNARE proteins on exocytosis from salivary glands
SNARE 蛋白的 siRNA 对唾液腺胞吐作用的影响
- 批准号:
17591945 - 财政年份:2005
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of exocytosis with adenovirus coexpression vector in salivary gland cells
唾液腺细胞中腺病毒共表达载体的胞吐作用分析
- 批准号:
15591973 - 财政年份:2003
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Adenovirus-mediated expression and functional analyses of SNARE proteins in salivery gland cells
腺病毒介导的唾液腺细胞中 SNARE 蛋白的表达和功能分析
- 批准号:
13671946 - 财政年份:2001
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular cloning and functional analysis of VAMP-2 binding proteins in parotid acinar cells.
腮腺腺泡细胞中 VAMP-2 结合蛋白的分子克隆和功能分析。
- 批准号:
11671849 - 财政年份:1999
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Role of Protein Phosphorylation in Amylase Exocytosis from the Parotid Gland.
蛋白质磷酸化在腮腺淀粉酶胞吐作用中的作用。
- 批准号:
01571024 - 财政年份:1989
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
Role of VAMP-2 and VAMP-7 in eosinophil degranulation in allergic inflammation
VAMP-2 和 VAMP-7 在过敏性炎症中嗜酸性粒细胞脱颗粒中的作用
- 批准号:
184121 - 财政年份:2009
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Studentship Programs
Molecular cloning and functional analysis of VAMP-2 binding proteins in parotid acinar cells.
腮腺腺泡细胞中 VAMP-2 结合蛋白的分子克隆和功能分析。
- 批准号:
11671849 - 财政年份:1999
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
耳下腺腺房細胞においてVAMP-2と相互作用するタンパク質の検索
寻找腮腺腺泡细胞中与 VAMP-2 相互作用的蛋白质
- 批准号:
09771550 - 财政年份:1997
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Encouragement of Young Scientists (A)