Imaging of chnages in intracellular ion distribution induced by receptor stimulation in parotid aciner cells

腮腺腺泡细胞受体刺激引起的细胞内离子分布变化的成像

• 批准号: 09671903
• 负责人: TOJYO Yosuke
• 金额: $ 2.05万
• 依托单位: Health Sciences University of Hokkaido, School of Dentistry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671903/
• 关键词: Parotid cells; Acinar cells; Intracellular Ca^<2+>; Calcium signal; Inositol-1,4,5-trisphosphate; Intracellular Ca^<2+> store; Ca^<2+> imaging; Ca^<2+>ウェーブ; イノシトール1,4,5-三リン酸; イソプロテレノール; β受容体; イメージング; カルシウムウェーブ; リアノジン受容体

1. Imaging of Ca^<2+> waves induced by muscarinic receptor stimulation. The fura-2 loaded rat parotid acinar cells were stimulated with calbachol (CCh). The increase in was initiated in the apical pole of the acinar cells and then rapidly spread as a Ca^<2+> wave towards the basolateral region.2. The Ca^<2+> wave in saponin-permeabilized parotid acinar cells evoked by flash photolysis of caged 1P3. The permeabilized acinar cells were labelled with the membrane-bound Ca^<2+> indicator Calcium Green C_<18> and stimulated by the photolysis of caged IP The Ca^<2+> release from intracellular stores started at the apical region and spread towards the basolateral region.3. Imaging of C^<2+> release from intracellular Ca^<2+> stores in permeabilized parotid acinar cells. The Ca^<2+> release from intracellular Ca^<2+> stores was monitored using the fluorescent indicators Mag-fura-2 and Calcium Green C_<18> App1ication of IP_3 caused a rapid release of Ca^<2+>, but none of cyclic ADP-ribose, caffeine or ryanodine stimulated Ca^<2+> release.4. Effect of isoproterenol on cytosolic Ca^<2+> distribution. Application of 1 muMisoproterenol, a concentration producing the maximum amylase release, did not cause any change in cytosolic Ca^<2+> distribution.5. Effect of CCh on cytosolic CI distribution. CCh caused a rapid dcrease in cytosolic CF concentration. This change was corelated with the increase in [Ca^<2+>].

1.毒蕈碱受体刺激诱导的Ca^<2+>波成像。用钙己醇（CCh）刺激负载Fura-2的大鼠腮腺腺泡细胞。结果：1.腺泡细胞内Ca^<2+>浓度的增加始于顶极，然后以Ca^<2+>波的形式迅速向基底外侧区扩散.笼状1 P3闪光光解诱发的皂化渗透性腮腺腺泡细胞Ca^<2+>波。用钙绿色C_标记膜结合的Ca^&lt;2+&gt;指示剂，<18>并通过笼状IP的光解刺激通透化的腺泡细胞，胞内钙库的Ca^&lt;2+&gt;释放开始于顶端区域，并向基底外侧区域扩散.透化腮腺腺泡细胞胞内Ca ^2+库释放C^2+的成像。用荧光指示剂Mag-fura-2和钙绿色C监测细胞内Ca ^&lt;2 +&gt;库的Ca <18>^&lt;2 +&gt;释放。异丙肾上腺素对细胞内Ca^&lt;2+&gt;分布的影响应用1 μ M异丙肾上腺素（产生最大淀粉酶释放的浓度）不会引起细胞质Ca^&lt;2+&gt;分布的任何变化。5. CCh对胞浆CI分布的影响。CCh引起胞浆CF浓度迅速下降。这种变化与[Ca^&lt;2+&gt;]的增加有关。

项目成果

Yosuke Tojyo: "Activation of β-adrenoceptors does not cause any change in cytosolic Ca2+ distribution in rat parotid acinar cells." Euro.J.Pharmacol.360(1). 73-79 (1998)

Yosuke Tojyo：“β-肾上腺素受体的激活不会导致大鼠腮腺腺泡细胞中胞质 Ca2+ 分布的任何变化。” Euro.J.Pharmacol.360(1) (1998)。

Yousuke Tojyo: "Imaging of intracellular Ca2+ waves induced by muscarinic receptor stimulauon in rat parotid acinar cells." Cell Calcium. 22(6). 455-462 (1997)

Yousuke Tojyo：“大鼠腮腺腺泡细胞中毒蕈碱受体刺激诱导的细胞内 Ca2 波成像。”

Akihiko Tanimura: "Polarized Ca2+ release in saponin-permeabilized parotid acinar cells evoked by flash photolysis of caged inositol 1, 4, 5-trisphosphate." Biochem.J.332(2). 769-772 (1998)

Akihiko Tanimura：“皂苷渗透性腮腺腺泡细胞中的极化 Ca2 释放是由笼状肌醇 1,4,5-三磷酸的闪光光解引起的。”

Yosuke Tojyo: "Digital imaging of intracellular Ca^<2+> signaling in rat parotid acinar cells" Life Sciences. 62・17/18. 1635-1639 (1998)

Yosuke Tojyo：“大鼠腮腺腺泡细胞内 Ca^<2+> 信号传导的数字成像”生命科学 62・17/18 (1998)。

Yusuke Tojyo: "Monitoring of Ca^<2+> release from intracellular stores in permiabilized rat parotid acinar cells using the fluorescent……" Biochem.Biophys.Res.Commn.240 (1). 189-195 (1997)

Yusuke Tojyo：“使用荧光技术监测大鼠腮腺腺泡细胞内储存的 Ca^<2+> 释放……”Biochem.Biophys.Res.Commn.240 (1) (1997)。