Molecular mechanism of the receptor-mediated Ca^<2+> entry in parotid cells

受体介导的Ca^2>进入腮腺细胞的分子机制

• 批准号: 07672023
• 负责人: TOJYO Yosuke
• 金额: $ 1.41万
• 依托单位: Health Sciences University of Hokkaido, School of Dentistry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672023/
• 关键词: Parotid cells; Intracellular Ca^<2+>; Capacitative Ca^<2+> entry; Intracellular Ca^<2+> stores; Kinase inhibitor; Phosphatase inhibitors; Purinergic receptor; カルシウム流入; 細胞内カルシウム動態; カルシウムチャンネル; プロテインキナーゼ; プロテイン・ホスファターゼ

1) Effects of the protein kinase inhibitor staurosporine on capacitative Ca^<2+> entry in rat parotid cells. Staurosporine significantly enhanced Ca^<2+> entry from extracellular medium activated by depletion of intracellular Ca^<2+> stores.2) Effects of serine/threonine phosphatase inhibitors on capacitative Ca^<2+> entry in rat parotid cells. The phosphatase inhibitors (calyculin-A, tautomycin, okadaic acid) suppressed the Ca^<2+> entry induced by depletion of intracellular Ca^<2+> stores.These results suggest that the capacitative Ca^<2+> entry may be regulated by protein phosphorylation/dephosphorylation.3) ATP-induced Ca^<2+> entry in rat parotid cells. Ectracellular ATP increased cytosolic Ca^<2+> concentration without formation of inositol phosphates. This increase appears to be due to an entry of Ca^<2+> via activation of P_<2Z> purinoceptors.4) Imaging of intracellular Ca^<2+> mobilizing in rat parotid acinar cells. When parotid acinus was stimulated with carbachol, the initial rise in cytosolic Ca^<2+> was detected in the apical pole of the cells. Thereafter, the increase spreaded into the basolateral area. When Ca^<2+> was added to the medium following depletion of intracellular Ca^<2+> stores, an increase in cytosolic Ca^<2+> was observed in the basal pole.5) Visualization of intracellular Ca^<2+> stores in a human parotid ductal cell line. The intracellular Ca^<2+> stores were visualized using digital imaging techniques with a confocal laser microscopy. The results demonstrate that the entire ER and the nuclear envelop serve as IP_3-sensitive Ca^<2+> stores.

1)蛋白激酶抑制剂星形孢菌素对大鼠腮腺细胞容量性钙内流的影响。2)丝氨酸/苏氨酸磷酸酶抑制剂对大鼠腮腺细胞容积性钙内流的影响。3)ATP诱导大鼠腮腺细胞内钙离子内流。这些结果提示，大鼠腮腺细胞的钙离子内流可能受蛋白磷酸化/去磷酸化的调节。胞外ATP可增加胞内Ca~(2+)~(2+)浓度，而不形成肌醇磷酸。这一增加似乎是由于钙离子通过激活P_2Z&gt；嘌呤受体进入细胞内。4)细胞内钙离子在大鼠腮腺腺泡细胞内动员的成像。用卡巴胆碱刺激腮腺腺泡细胞，细胞顶端的胞浆Ca~(2+)~(2+)和Gt~(2+)开始升高。此后，这种增长蔓延到了基底外侧地区。当细胞内钙离子储备耗尽后加入培养液中，基极细胞内钙离子储备增加。5)人腮腺导管细胞系细胞内钙离子储备的可视化。用激光共聚焦显微镜的数字成像技术显示细胞内的钙离子储存。结果表明，整个内质网和核膜是IP_3敏感的Ca~(2+)&gt；

项目成果

東城庸介: "唾液腺-耳下腺房細胞における分泌機能と情報伝達" 生体の科学. 46(3). 278-285 (1995)

Yosuke Tojo：“唾液腺-腮腺腺泡细胞的分泌功能和信息传递”《生物科学》46（3）（1995）。

Yosuke Tojyo: "Suppression of capacitative Ca^<2+> entry by scrine/threonine phopsphatase inhibitors in rat parotid acinar cells" Japan,J.Pharmacol.64(4). 381-389 (1995)

Yosuke Tojyo：“大鼠腮腺腺泡细胞中丝氨酸/苏氨酸磷酸酶抑制剂对电容性Ca^2进入的抑制”日本，J.Pharmacol.64(4)。

Yosuke Tojyo: "Suppression of capacitative Ca^<2+> entry by serine/threonire phosphatase inhibitors in rat parotid acinar cells" Jpn. J. Pharmacol.69. 381-389 (1995)

Yosuke Tojyo：“大鼠腮腺腺泡细胞中丝氨酸/苏氨酸磷酸酶抑制剂对电容性Ca ^ 2 进入的抑制”Jpn。

Yosuke Tojyo: "Effects of extracellular ATP on cytosolic Ca^<2+> concentration and secretory responses in rat parotid acinar cells" Archs Oral Biol.(in press). (1997)

Yosuke Tojyo：“细胞外ATP对大鼠腮腺腺泡细胞中胞浆Ca 2+ 浓度和分泌反应的影响”Archs Oral Biol。（正在印刷中）。

Yosuke Tojyo: "Effects of extracellular ATP on cytosolic Ca^<2+> concentration and secretory responses in rat parotid acinar cells" Archives of Oral Biology. (in press).

Yosuke Tojyo：“细胞外ATP对大鼠腮腺腺泡细胞胞浆Ca 2+ 浓度和分泌反应的影响”口腔生物学档案。