Study on measurement of infectious environmental contamination of dental office-Hygienic management with the use of Adenosine Tri-Phosphate as a contamination index-

牙科诊所感染性环境污染测定研究-以三磷酸腺苷为污染指标的卫生管理-

• 批准号: 09672122
• 负责人: KASAMO Yukitsugu
• 金额: $ 2.11万
• 依托单位: NIHON UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672122/
• 关键词: dental office; infectious environmental contamination; Adenosine Tri-Phosphate; contamination index; hygienic management (hygienics); swab-rinse method; luciferin-luciferase; luminescence; 拭き取り検査; ATP; ルシフェラーゼ; 汚染度; 感染予防

"Swab-rinse method" has been established with the use of ATP to measure the contamination of dental office environment. As a contamination index, ATP measurement was taken by luminescence with a luminometer (lumitester K-210) on the solution made of lOOmul of specimen liquid and lOOmul of ATP extract reagent with addition of ATP luminescent reagent (luciferin - luciferase) Areas measured were tip and handle portions of instruments and equipment, the face and chest surface of the operator, assistant and patient, and left and right forearms of the operator and assistant.The lower the ATP concentration ( = the degree of pollution) is, the lower the luminescent measurement value (RLU) is. The degree of contamination was measured in subject areas for inspection with a reference value of sterilized distilled water (less than 10) . As a result, values over 10^4 were recorded with the head of high-speed handpiece, HVE tip and scaler. Some of the faces of the operators and patients exceeded l0^3. Some cases showed over 10^2 in the forearms of the operator and assistant. In the food manufacturing and processing industry, " Cleanliness of cutting board" is reportedly the standard to determine the safety, which is less than 10^2, however, there is little chance for microbes to be detected at the luminescent level of less than 10^2. Cutting dust produced in the dental practice, on the other hand, contains various kinds and amounts of bacteria and viruses in blood and saliva from the mouths of patients, and it is quite difficult to identify these microbes by culturing. Therefore, it is considered to be necessary to tighten the safety standard to prevent from occurrence of secondary infection.

建立了用三磷酸腺苷（ATP）测定牙科诊室环境污染的“拭子-冲洗法”。作为污染指标，ATP测量通过使用发光计的发光进行（Lumitester K-210）对由10 μ l样品液和10 μ l ATP提取试剂制成的溶液进行荧光检测，（荧光素酶）测量的区域是仪器和设备的尖端和手柄部分，操作者、助手和患者的面部和胸部表面，ATP浓度（=污染程度）越低，发光测定值（RLU）越低。以无菌蒸馏水为参考值（小于10），测量受检区域的污染程度。结果，使用高速手机头部、HVE尖端和洁牙机记录的数值超过10^4。一些操作者和患者的面部超过l0^3。有些病例显示操作者和助手前臂的10^2以上。在食品制造和加工行业中，“砧板清洁度”据说是确定安全性的标准，其小于10^2，然而，在小于10^2的发光水平下检测微生物的机会很小。另一方面，在牙科实践中产生的切割粉尘在来自患者口腔的血液和唾液中含有各种类型和数量的细菌和病毒，并且很难通过培养来识别这些微生物。因此，认为有必要加强安全标准，以防止发生继发感染。