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Evaluation method of infectiosity pollution by the air-born bacteria in dentistry clinic.

牙科诊所空气传播细菌传染性污染评价方法

基本信息

批准号：
09672121
负责人：
SUYAMA Yuji
金额：
$ 1.86万
依托单位：
Tokyo Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Blood and drill dust from dental plaque microorganisms, teeth, and filling materials can cause environmental polllution in the dental clinic. Currently, as a preventive measure against air pollution from a patient's mouth during dental treatment. Detetion of oral streptococci in the air-borne is used as an index on the presence of aerosol in consequence of the use of tooth preparation with high-speed air turbines. The air-borne bacteria in the dental clinics consisted of Guram-positive cocci(33%), Guram-positive bacilli(37%), Myces(38%). We recognized 30.6% oral-α,γ-haemolytic streptococci according to the growth shown on Mitis-Sali varius agar disk, while 4.5% recognized on blood agar disk. But we could not find oral-α-γ haemolytic streptococci from the airborne bacterium in the denal clinic's waiting room and in the research room. Oral-α-γ haemolytic streptococci using Mitis Salivarius ager showed more efficiency as the index in monitoring droplet infection at dental treatments and pollution of the in-clinic environment, thus considered as an efficient procedure in detection environmental pollution in the dental clinic. With detection of oral-α-γ haemolytic streptococci two hours after tooth-drilling, necessity of monitoring on standing of air-borne bacteria was considered. We tested the eliminating effects by the air-cleaning system (CLEAN PARTITON) with the plaque solution aerosol and the aerosol from drilling a tooth by examing the distriution of floating aerosol in the air turbine's tank when a plaque solution was sprayed and when a human tooth was drilled with a plaque solution.

来自牙菌斑微生物、牙齿和填充材料的血液和钻尘可导致牙科诊所的环境污染。目前，作为防止牙科治疗期间来自患者口腔的空气污染的预防措施。在空气传播中口腔链球菌的检测被用作使用高速空气涡轮机进行牙准备后是否存在气溶胶的指标。口腔门诊空气中细菌主要为古兰氏阳性球菌（33%）、古兰氏阳性杆菌（37%）、霉菌（38%）。根据在Mitis-Sali Varius琼脂平板上的生长情况，我们识别出30.6%的口服α，γ-溶血性链球菌，而在血琼脂平板上识别出4.5%的口服α，γ-溶血性链球菌。但在门诊候诊室和研究室均未发现来自空气传播细菌的口腔-α-γ溶血性链球菌。口腔α-γ溶血性链球菌的检测结果表明，唾液螨法检测口腔α-γ溶血性链球菌在监测口腔治疗过程中的飞沫感染和口腔环境污染方面具有较高的效率，可作为口腔环境污染监测的一种有效方法。通过对牙钻后2 h口腔α-γ溶血性链球菌的检测，认为有必要对空气传播菌群进行监测。我们通过检测空气涡轮机槽中悬浮气溶胶的分布，测试了空气净化系统（CLEAN PARTITON）对菌斑溶液气溶胶和牙钻气溶胶的清除效果。