The functions and their mechanisms of DNA-binding proteins, HMG1 and HMG2

DNA结合蛋白HMG1和HMG2的功能及其机制

• 批准号: 09680601
• 负责人: YOSHIDA Michiteru
• 金额: $ 2.18万
• 依托单位: Science University of Tokyo, Department of Biological Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680601/
• 关键词: HMG proteins; nuclear chromosomal protein; HMG1; HMG2; chromatin structure; DNA-protein interaction; DNA結合タンパク質; 転写促進; DNA結合ドメイン構造; 表面プラズモン共鳴; cDNAクローニング

(1) The mechanism of DNA-binding of HMG 1/2 proteins and the accompanied changes in DNA conformation : The respective single HMG box in HMG2 protein has DNA-binding activity. The tandem array of the two HMG boxes strengthens the activity. One of the major factor of the stronger DNA-binding activity of box B is resulted from the intercalation of side chain of F107. Computer simulation for HMG2 protein structure showed a structural model in which two boxes are linked in head-to-head manner.The major functional region in HMG2 protein for its preferential binding with supercoiled DNA, DNA unwinding activity, and DNA protection activity from nuclease S1 digestion is in box B flanked by the neighboring sequences.(2) The functions of HMG1/2 proteins : The functional analyses for HMG proteins 1 and 2 revealed that these proteins have multiple functions concerned in transcription, DNA replication, DNA recombination, DNA repair and DNA integration of outer DNA into chromosomal DNA.

(1)HMG 1/2蛋白的DNA结合机制和伴随的DNA构象变化：HMG2蛋白中各自的单个HMG框具有DNA结合活性。两个 HMG 盒的串联阵列增强了活性。 Box B 更强的 DNA 结合活性的主要因素之一是 F107 侧链的插入所致。 HMG2蛋白结构的计算机模拟显示了一个结构模型，其中两个盒以头对头的方式连接。HMG2蛋白的主要功能区位于盒B中，其优先与超螺旋DNA结合、DNA解旋活性和核酸酶S1消化时的DNA保护活性。(2)HMG1/2蛋白的功能：HMG的功能分析 蛋白质1和2表明这些蛋白质具有多种功能，涉及转录、DNA复制、DNA重组、DNA修复和外部DNA整合到染色体DNA中。

项目成果

Shirakawa, H., Tanigawa, T., Kobayashi, M., Terashima, T., Yoshida, K., Arai, T.and Yoshida, M.: "Nuslear accumulation of HMG2 protein is mediated by basic regions interspaced with a long DNA-binding sequence, and retention within the nucleus requires the

Shirakawa, H.、Tanikawa, T.、Kobayashi, M.、Terashima, T.、Yoshida, K.、Arai, T. 和 Yoshida, M.：“HMG2 蛋白的核积累是由长链间隔的基本区域介导的。

Sobajima J., et al.: "Prevalence and chracterization of perinuclear anti-neutrophil cytoplasmic antibodies directed against HMG1 and 2 in ulcerative" Clin. Exp. Immunol.111. 402-407 (1998)

Sobajima J. 等人：“溃疡病中针对 HMG1 和 2 的核周抗中性粒细胞胞质抗体的流行和特征”Clin。

Yumoto Y., et al.: "High mobility group proteins 1 and 2 can function as DNA-binding regulatory components for DNA-dependent protein kinase in vitro." J.Biochem.124. 519-527 (1998)

Yumoto Y. 等人：“高迁移率族蛋白 1 和 2 可以在体外作为 DNA 依赖性蛋白激酶的 DNA 结合调节成分。”

Nagaki S., et al.: "Non-histone chromosomal proteins HMG1 and 2 enhance ligation reaction of DNA double-strand breaks." Biochem.Biophys.Res.Commun.246. 137-141 (1998)

Nagaki S. 等人：“非组蛋白染色体蛋白 HMG1 和 2 增强 DNA 双链断裂的连接反应。”

Nagaki, S., Yamamoto, M., Yumoto, y., Shirakawa, H., Yoshida, M.and Teraoka, H.: "Non-histone chromosomal proteins HMG1 and 2 enhance ligation reaction of DNA double-strand breaks." Biochem.Biophys.Res.Commun.246. 137-141 (1998)

Nagaki, S.、Yamamoto, M.、Yumoto, y.、Shirakawa, H.、Yoshida, M.和 Teraoka, H.：“非组蛋白染色体蛋白 HMG1 和 2 增强 DNA 双链断裂的连接反应。”