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病的血管に対する人工血管移植時の吻合部治癒機転と器質化に関する研究

病理血管人工血管移植时吻合口愈合机制及组织研究

基本信息

批准号：
09877224
负责人：
佐久間まこと
金额：
$ 1.22万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Exploratory Research
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

[目的]病的血管に人工血管を移植した後の吻合部創傷治癒、器質化特性をCollagen動態、とくに抗Collagen抗体や抗α-Smooth muscle actin抗体、Splraled Collaagenとmetalloproteinase(MMP)発現の観点から検討して、人工血管の病的状態下での治癒特性の特徴を把握して吻合部合併症の発生予防のための資となすことを目的とした。[方法](1)人工血管移植モデルとして雑種成犬の頚動脈・大腿動脈に口径4mmの人工血管を移植する(2)移植後1〜7日の急性期および4週以降の慢性期に血管吻合部を含めて人工血管を摘出し、比色定量法によるTypeI〜VCollagenの測定と各タイプの抗Collagen抗体の発現を観察すると共に、人工血管の吻合部創治癒と器質化特性に関与する抗Collagen抗体、抗αSmooth muscleactin抗体、I、III型Collagenを分解するMMP-1との関連性を比較した。小口径人工血管(ePTFE,4mm径)を雑種成犬5頭の頚動脈(合計10本)と置換して経時的にこれを摘出し、吻合部を中心にCollagen typeIおよびIIIと抗Collagen抗体および抗α-Smlooth muscleactin抗体、MMP-1の発現を免疫染色にて検討した。[結果とまとめ]人工血管は全例開存しており、4週以降では吻合部を中心に仮性内膜の形成が認められた。抗Collagen抗体および抗α-Smooth muscleactin抗体染色では4週および16週でとくに抗α-Smooth muscle actin抗体の発現が吻合部において強く認められた。吻合部治癒過程における平滑筋細胞の増殖が人工血管の器質化に関連していることが推測された。MMP-1は吻合部近傍で4週および8週の時点でわずかに発現を認めたが、抗Collagen抗体、抗αSmooth muscleactin抗体との関連性は明らかではなく、治癒過程で平滑筋細胞の増殖が活発に起こるモデルではMMPの発現は抑制されている可能性が考えられた。

[objective] after the transplantation of diseased vascular prosthesis, the anastomotic site was treated, Collagen dynamics, anti-Collagen antibody, anti-α-Smooth muscle actin antibody, Splraled colagenated metalloproteinase (MMP) were detected, and the clinical characteristics of vascular prosthesis disease were observed. [methods] (1) artificial vascular transplantation (1) artificial vascular transplantation was performed in adult dogs. Thigh exercise caliber 4mm artificial vascular graft was used. (2) during the acute period of 1-7 days after transplantation, the vascular anastomosis in the chronic phase was reduced. The artificial vascular graft was extracted and the anti-Collagen antibody was detected by colorimetric quantitative TypeI~VCollagen assay. The chemical characteristics of the anastomotic site of the artificial blood vessel were compared with those of anti-Collagen antibody, anti-α Smooth muscleactin antibody, type I and III Collagen antibody, MMP-1 antibody and connectivity. Small caliber artificial blood vessels (ePTFE, 4mm diameter) were used in 5-year-old adult dogs (a total of 10 copies). In the center of the anastomotic part, the anti-α-Smlooth muscleactin antibody was detected by III anti-Collagen antibody in the center of the anastomosis, and the immune staining was detected by MMP-1 immunostaining. [results] in all cases, the artificial blood vessels were stored for 4 years to reduce the abnormal intima in the center of the anastomotic site. Anti-Collagen antibody, anti-alpha-Smooth muscleactin antibody staining, anti-alpha-Smooth muscleactin antibody staining, anti-alpha-Smooth muscleactin antibody staining, anti-alpha-Smooth muscleactin antibody staining. The anastomotic part is used to treat the smooth tendon cell to proliferate the artificial blood vessel. The MMP-1 anastomosis site was used to detect antigens, anti-Collagen antibodies, anti-α Smooth muscleactin antibodies in the anastomotic part of the anastomosis at different times, and to detect the possibility of inhibiting the activity of antigens.