'Cradle to the grave' life histories of every bacterium link individual metabolic activity to population activity

每种细菌的“从摇篮到坟墓”的生命史将个体代谢活动与群体活动联系起来

• 批准号: 22K18368
• 负责人: UTADA ANDREW
• 金额: $ 16.56万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Research (Pioneering)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-06-30 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K18368/
• 关键词: heterogeneity; metabolism; NanoSIMS; Single-cell tracking; Metaboloic activitity; Bacteria; Heterogeneity

In the past year, we have started by finding an appropriate research scientist. We have begun to identify a set of bacteria to use that are genetically tractable and widely applicable. This list currently includes Escherichia coli and Pseudomonas aeruginosa. We have focused on developing the two major thrusts of our targeted work: single cell tracking in confined conditions and the nano secondary ion mass spectrometry (nanoSIMS) process. We have already begun to optimized our cell tracking using publicly available machine learning algorithms combined with custom designed microfluidic chambers.Simultaneously, we have developed microfabrication methods to use with hard glass substrates. We plan to combine these results soon to use with the local nanoSIMS facility.

在过去的一年里，我们首先找到了一位合适的研究科学家。我们已经开始确定一组可以使用的细菌，这些细菌在基因上是易驯服的，并且广泛适用。这份名单目前包括大肠埃希菌和铜绿假单胞菌。我们专注于开发我们目标工作的两个主要推动力：受限条件下的单细胞跟踪和纳米二次离子质谱仪(NanSIMS)过程。我们已经开始使用公开可用的机器学习算法结合定制设计的微流体室来优化我们的细胞跟踪。同时，我们已经开发了用于硬玻璃基板的微制造方法。我们计划很快将这些结果与当地的NanSIMS设施结合使用。