'Cradle to the grave' life histories of every bacterium link individual metabolic activity to population activity

在过去的一年里，我们开始寻找合适的研究科学家。我们已经开始确定一套细菌使用，是遗传驯服和广泛适用。该列表目前包括大肠杆菌和铜绿假单胞菌。我们专注于开发我们的目标工作的两个主要推动力：在受限条件下的单细胞跟踪和纳米二次离子质谱（nanoSIMS）过程。我们已经开始使用公开的机器学习算法结合定制设计的微流体室来优化我们的细胞跟踪。同时，我们开发了用于硬玻璃基板的微加工方法。我们计划很快将联合收割机这些结果与当地的纳米SIMS设施结合使用。

In the past year, we have started by finding an appropriate research scientist. We have begun to identify a set of bacteria to use that are genetically tractable and widely applicable. This list currently includes Escherichia coli and Pseudomonas aeruginosa. We have focused on developing the two major thrusts of our targeted work: single cell tracking in confined conditions and the nano secondary ion mass spectrometry (nanoSIMS) process. We have already begun to optimized our cell tracking using publicly available machine learning algorithms combined with custom designed microfluidic chambers.Simultaneously, we have developed microfabrication methods to use with hard glass substrates. We plan to combine these results soon to use with the local nanoSIMS facility.