Chemically Modified Nanoporous Titanium Oxide for Biomedical Applications

由于钛和钛合金具有很高的生物相容性，目前作为优异的生物医学植入材料应用于医疗技术。修饰表面化学和表面氧化物的纳米级结构对于这种植入物在人或动物体内的生存环境中的整合和生物相容性具有至关重要的意义。本项目拟使用多孔氧化钛层（特别是垂直取向的TiO2纳米管层），并旨在将自组装有机单层（SAMs）与细胞粘附蛋白、粘附肽和生长因子（细胞生长支持分子= CGSM）作为表面涂层接枝，以修饰表面化学和形态，优化材料的生物相容性。特别是，我们将利用纳米管的开放体积来实现细胞生长支持因子的高填充密度。细胞对纳米多孔氧化钛表面的粘附、扩散、形态、增殖和分化等反应将通过建立的体外方法进行研究。这些改性的氧化钛表面将用于空间限制，排列和引导细胞，通过创建一个化学和结构上定义的细胞生物系统，细胞相互作用和细胞活动可以被监测和控制。从这些研究中获得的信息将对工业应用和发展产生即时影响，特别是在生命科学行业生产高度生物相容性的钛表面，用作骨科和牙科植入物在替代手术中。

Due to their high biocompatibility, titanium and titanium alloys are currently used in medical technology as superior biomedical implant materials. Modifications of the surface chemistry and the nanoscale structure of the surface oxide are of crucial significance for integration and biocompatibility of such implants into living environments of human or animal bodies. The present project intends to use porous titanium oxide layers (particularly vertically orientated TiO2 nanotube layers) and aims at grafting self - assembled organic monolayers (SAMs) with cell adhesive proteins, adhesive peptides and growth factors (cell growth supporting molecules = CGSM) as surface coatings in order to modify surface chemistry and morphology for optimizing the biocompatibility of the material. In particular we will exploit the open volume of the nanotubes to achieve a high filling density of cell growth supporting factors. Cellular responses to the coated nanoporous titanium oxide surfaces such as adhesion, spreading, morphology, proliferation and differentiation will be investigated with established in vitro methods. These modified titanium oxide surfaces will be used to spatially confine, align and guide cells by creating a chemically and structurally defined cellular biosystem where cell interactions and cellular activities can be monitored and controlled. The information gained from these studies will have an instantaneous impact on industrial application and development, in particular in life sciences industry for the production of highly biocompatible surfaces on titanium used as orthopedic and dental implants in replacement surgery.