Correlation between physical stability map and genetic map of DNA double strand

DNA双链物理稳定性图与遗传图的相关性

• 批准号: 59420051
• 负责人: WADA Akiyoshi
• 金额: $ 9.92万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1985
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59420051/
• 关键词: DNA; stability map; A-DNA; B-DNA; codon; correlation; database; genetic map; 遺伝子地図

The purpose of this project is to reveal the correlation between physical stability map and genetic map of the DNA molecular thread. As the physical stability map, we have studied those in the B-A transition and the helix-coil transition.The B-A transition of ColE1 DNA caused by high ethanol concentrations has been studied by the multi-dimensional spectrophotometer equipped with the computer-controlled microbullet. The transition is broader than that of alternative copolymers with shorter lengths and varies among restriction fragments. These facts have provided the first evidence to prove that the stability in the B-A transition varies along a long DNA double strand.Comparison between the double-helix stability distribution and the location of genes in a large number of DNAs has disclosed the "gene homostabilizing propensity": the tendency for each protein coding region to have a uniform stability distribution around its own characteristic value. The propensity was found to be attained by the synonymous codon usage. The redundancy at the wobble third base is utilized to counterbalance the local G+C content variation due to the first and second bases of codons.

该项目的目的是揭示物理稳定性图与DNA分子线的遗传图之间的相关性。作为物理稳定性图，我们已经研究了B-A转变和螺旋线圈​​过渡中的那些。COLE1DNA的B-A转变由高乙醇浓度引起的B-A过渡已通过配备了计算机控制的Microbullet的多维分光光度计研究了高乙醇浓度。过渡比限制片段之间的替代共聚物的过渡更广泛。这些事实提供了第一个证据，以证明BA过渡的稳定性随长的DNA双重链条而变化。双螺旋稳定性分布与大量DNA中基因位置之间的比较揭示了“基因均匀稳态倾向”：每个蛋白质编码区域的趋势均具有其自身特征性的统一稳定性。发现倾向是通过同义密码子的使用来实现的。由于密码子的第一基地和第二基础，Wobble第三键的冗余用于抵消本地G+C含量变化。

项目成果

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