Correlation between physical stability map and genetic map of DNA double strand

DNA双链物理稳定性图与遗传图的相关性

• 批准号: 59420051
• 负责人: WADA Akiyoshi
• 金额: $ 9.92万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1985
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59420051/
• 关键词: DNA; stability map; A-DNA; B-DNA; codon; correlation; database; genetic map; 遺伝子地図

The purpose of this project is to reveal the correlation between physical stability map and genetic map of the DNA molecular thread. As the physical stability map, we have studied those in the B-A transition and the helix-coil transition.The B-A transition of ColE1 DNA caused by high ethanol concentrations has been studied by the multi-dimensional spectrophotometer equipped with the computer-controlled microbullet. The transition is broader than that of alternative copolymers with shorter lengths and varies among restriction fragments. These facts have provided the first evidence to prove that the stability in the B-A transition varies along a long DNA double strand.Comparison between the double-helix stability distribution and the location of genes in a large number of DNAs has disclosed the "gene homostabilizing propensity": the tendency for each protein coding region to have a uniform stability distribution around its own characteristic value. The propensity was found to be attained by the synonymous codon usage. The redundancy at the wobble third base is utilized to counterbalance the local G+C content variation due to the first and second bases of codons.

本项目的目的是揭示DNA分子链的物理稳定性图谱与遗传图谱之间的相关性。作为物理稳定性图谱，我们研究了B-A跃迁和螺旋-卷曲跃迁的物理稳定性，并利用配有计算机控制的微弹的多维分光光度计研究了高浓度乙醇引起的ColE 1 DNA的B-A跃迁。该过渡比具有较短长度的替代共聚物的过渡更宽，并且在限制性片段之间变化。这些事实首次证明了B-A转换的稳定性是沿着一条长DNA双链变化的，通过对大量DNA的双螺旋稳定性分布与基因位置的比较，揭示了“基因同源稳定化倾向”：即每个蛋白质编码区在其自身特征值附近具有均匀的稳定性分布的倾向。发现这种倾向是通过同义密码子的使用来实现的。利用摆动第三碱基的冗余来平衡由于密码子的第一和第二碱基引起的局部G+C含量变化。

项目成果

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