Transmission of visual information in rod outer segments.
在杆外段传输视觉信息。
基本信息
- 批准号:59440003
- 负责人:
- 金额:$ 17.92万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (A)
- 财政年份:1984
- 资助国家:日本
- 起止时间:1984 至 1985
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Several steps of Photo-transduction process in rod outer segments were investigated by use of spectroscopic and biochemical techniques.1) Photochemical reaction of rhodopsin was investigated by means of picosecond laser photolysis. New bathochromic photoproduct "photorhodopsin", which was a precursor of bathorhodopsin, was found by excitation of rhodopsin with picosecond weak pulse. It was clarified that the reason why photorhodopsin was not detected in the preceding experiments was due to excitation of a rhodopsin sample with highly intense laser pulse.2) Deuterium effects on the formation and decay processes of photorhodopsin were investigated, where no deuterium effect was observed.3) Photo-isomerization of the chromophore of rhodopsin was investigated by use of low temperature spectrophotometry with retinal analogues. It was clarified that the isomerization of the retinylidene chromophore occured without any movement of its <beta> -ionone ring in the chromophore binding site of opsin and the conversion of bathorhodopsin to lumirhodopsin was induced by displacement of the <beta> -ionone ring.4) Photo-affinity labeling of <^3H> -cGMP against the rod outer segments showed that integral membrane proteins of molecular weights 100 K and 92 K were specifically labeled with cGMP. This result suggests that these proteins may be candidates of channel proteins in rod plasma membrane.5) cGMP-dependent phosphorylated proteins were purified from rod outer segments by column chromatography. Physiological role of the phosphorylation of the proteins was suggested to be intensification of activity of cGMP phosphodiesterase.6) Monoclonal antibodies of iodopsin and halorhodopsin were prepared.
用光谱学和生物化学技术研究了杆状外段的几个光传导过程。1)用皮秒激光光解研究了视紫红质的光化学反应。用皮秒弱脉冲激发视紫红质,发现了一种新的赤致变色光产物“光紫质”,它是底色紫质的前体。解释了在前面的实验中没有检测到光紫质的原因是由于高强度激光脉冲激发视紫红质样品所致。2)研究了氘对光紫质形成和衰变过程的影响,其中没有观察到氢效应。3)利用视网膜类似物的低温分光光度法研究了视紫红质生色团的光异构化。结果表明,视黄亚甲基发色团的异构化反应没有发生在视黄素的发色团结合部位,紫罗兰酮环没有发生任何移动,底视紫红质转化为光紫质是通过紫罗兰酮环的位移而发生的。4)~lt;这一结果提示这些蛋白可能是视杆细胞质膜上的通道蛋白。5)柱层析从视杆细胞外段中纯化了依赖cGMP的磷酸化蛋白。蛋白质磷酸化的生理作用可能是通过增强cGMP磷酸二酯酶的活性来实现的。6)制备了碘视黄素和卤视紫红质的单抗。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
YOSHIZAWA Toru其他文献
YOSHIZAWA Toru的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('YOSHIZAWA Toru', 18)}}的其他基金
Development of a 3D endoscope system with higher function
开发更高功能的3D内窥镜系统
- 批准号:
20500398 - 财政年份:2008
- 资助金额:
$ 17.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study on Fabrication of 3D Modeling by Photo-Hardening Process based on 3D Shape Measurement Data
基于 3D 形状测量数据的光硬化工艺制造 3D 模型的研究
- 批准号:
05650108 - 财政年份:1993
- 资助金额:
$ 17.92万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Studies of New Opto-Mechatronics
新型光机电一体化研究
- 批准号:
04045022 - 财政年份:1992
- 资助金额:
$ 17.92万 - 项目类别:
Grant-in-Aid for international Scientific Research
Dynamics of Biomolecules in Photoreceptor Cells of Twilight and Color Vision
暮光和色觉感光细胞生物分子的动力学
- 批准号:
63065002 - 财政年份:1988
- 资助金额:
$ 17.92万 - 项目类别:
Grant-in-Aid for Specially Promoted Research
Molecular Mechanisms of Phototransduction in Visual Cells.
视觉细胞光转导的分子机制。
- 批准号:
60304098 - 财政年份:1985
- 资助金额:
$ 17.92万 - 项目类别:
Grant-in-Aid for Co-operative Research (A)
相似国自然基金
Rhodopsin跨纤毛运输与微丝组装复合体在光感受器膜盘生物发生过程的功能关系分析
- 批准号:32371015
- 批准年份:2023
- 资助金额:50 万元
- 项目类别:面上项目
基于CRISPR/Cpf1的Rhodopsin突变型视网膜色素变性基因治疗研究
- 批准号:81600770
- 批准年份:2016
- 资助金额:20.0 万元
- 项目类别:青年科学基金项目
固体核磁共振解析七次跨膜蛋白Leptosphaeria rhodopsin的三维结构
- 批准号:31470727
- 批准年份:2014
- 资助金额:95.0 万元
- 项目类别:面上项目
Rhodopsin-like GPCR参与棉蚜解毒相关P450基因表达的调控机制
- 批准号:31402024
- 批准年份:2014
- 资助金额:24.0 万元
- 项目类别:青年科学基金项目
膜蛋白Leptosphaeria rhodopsin二聚化组装的结构机制研究
- 批准号:21273279
- 批准年份:2012
- 资助金额:68.0 万元
- 项目类别:面上项目
视黄醛蛋白Leptosphaeria Rhodopsin中的质子跨膜传递机制的研究
- 批准号:31170794
- 批准年份:2011
- 资助金额:46.0 万元
- 项目类别:面上项目
基于空间定位顺磁共振技术对膜蛋白Leptosphaeria Rhodopsin的结构与跨膜传递氢离子机制的研究
- 批准号:31070662
- 批准年份:2010
- 资助金额:8.0 万元
- 项目类别:面上项目
相似海外基金
Exploring Microbial Light-Harvesting with Rhodopsin in Extreme Polar Environments: Unveiling Distribution, Diversity, and Functional Insights
在极端极地环境中探索利用视紫红质进行微生物光捕获:揭示分布、多样性和功能见解
- 批准号:
24K03072 - 财政年份:2024
- 资助金额:
$ 17.92万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Physiological function of microbial rhodopsin in the regulation of plant growth by soil bacteria
微生物视紫红质在土壤细菌调控植物生长中的生理功能
- 批准号:
23K05007 - 财政年份:2023
- 资助金额:
$ 17.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular -Scale Mapping of Rhodopsin Trafficking in Mammalian Rod Photoreceptors
哺乳动物视杆光感受器中视紫红质运输的分子尺度图谱
- 批准号:
10593138 - 财政年份:2022
- 资助金额:
$ 17.92万 - 项目类别:
Structural and functional analysis of novel transporter-type rhodopsin
新型转运蛋白型视紫红质的结构和功能分析
- 批准号:
22K19265 - 财政年份:2022
- 资助金额:
$ 17.92万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Molecular -Scale Mapping of Rhodopsin Trafficking in Mammalian Rod Photoreceptors
哺乳动物视杆光感受器中视紫红质运输的分子尺度图谱
- 批准号:
10334877 - 财政年份:2022
- 资助金额:
$ 17.92万 - 项目类别:
Elucidation of the mechanism of rhodopsin functions for optogenetics
阐明视紫质的光遗传学功能机制
- 批准号:
21H04969 - 财政年份:2021
- 资助金额:
$ 17.92万 - 项目类别:
Grant-in-Aid for Specially Promoted Research
Pharmacological studies of rhodopsin metabolism
视紫红质代谢的药理学研究
- 批准号:
10653840 - 财政年份:2020
- 资助金额:
$ 17.92万 - 项目类别:
Pharmacological studies of rhodopsin metabolism
视紫红质代谢的药理学研究
- 批准号:
10438670 - 财政年份:2020
- 资助金额:
$ 17.92万 - 项目类别:
A rhodopsin-based two-photon voltage indicator for all-optical mapping of synaptic connectivity in vivo
基于视紫红质的双光子电压指示器,用于体内突触连接的全光映射
- 批准号:
442616457 - 财政年份:2020
- 资助金额:
$ 17.92万 - 项目类别:
Research Fellowships
Pharmacological studies of rhodopsin metabolism
视紫红质代谢的药理学研究
- 批准号:
10224916 - 财政年份:2020
- 资助金额:
$ 17.92万 - 项目类别: