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Dynamics of Biomolecules in Photoreceptor Cells of Twilight and Color Vision

暮光和色觉感光细胞生物分子的动力学

基本信息

批准号：
63065002
负责人：
YOSHIZAWA Toru
金额：
$ 121.6万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Specially Promoted Research
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

Following progresses have been made. [1] Resonance Raman spectroscopic study on 7, 9-dicis-rhodopsin demonstrated that the Schiff base linkage between its chromophore (7, 9-dicis-retinal) and bovine opsin retained anti form as in the case of rhodopsin. [2] A comparison of photoreactions between rhodopsin and 7-cis-rhodopsin revealed that an interaction between opsin and the 9-methylgroup of the chromophore changed during the lumiーmeta I transition. [3] Analysis of the photoreactions of synthetic rhodopsin analogs indicated that a conformational change around betaーionone ring of the chromophore should occur during bathoーlumi transition. [4] Laser flash photolysis of 8-memberedーrhodopsin showed that photoーbatho transition process corresponded to a relaxing pathway of highly twisted conformation of trans C_<11>-C_<12> double bond in the chromophore. [5] FTIR spectroscopy showed that the Schiff base of hypsorhodopsin was protonated. [6] In the photocycle of bacteriorhodopsin, an increase o … More f interaction between the protein moiety and the protonated Schiff base of the retinylidene chromophore was observed at the stages of both L and N intermediates by FTIR spectroscopy. [7] FTIR spectroscopy showed that Asp-96 in N intermediated of bacteriorhodopsin was deprotonated while Asp-85 was protonated. [8] Chromophore extraction experiments indicated that the photobleaching of iodopsin was initiated by the cis-trans isomerization of C_<11>-C_<12> double bond in the retinylidene chromophore. [9] It was suggested that the lifetime of metaiodopsin II would be extremely shorter than that of metarhodopsin II. [10] Analysis of epitope (s) of our monoclonal antibodies specific for iodopsin is now being investigated. The C-terminal part of iodopsin could be the antigenic ite. [11] A photobleaching intermediate of iodopsin was phosphorylated by purified bovine rhodopsin kinase. [12] A candidate protein for putative cone-specific G-protein alpha-subunit was partially purified from chicken retinas. [13] Amino acid sequence of iodopsin was deduced from its complementary DNA. Cloning of the other cone pigments in a chicken retina is now under progress. Less

取得了以下进展。[1]用共振拉曼光谱研究了7，9-二-视紫红质，发现其发色团（7，9-二-视黄醛）与牛视蛋白之间的席夫碱键与视紫红质一样保持反式。[2]视紫红质和7-顺式视紫红质之间的光反应的比较表明，视蛋白和9-甲基基团的发色团之间的相互作用改变在Lumi-间Meta I过渡。[3]对合成的视紫红质类似物的光反应的分析表明，在batho pallumi转变过程中，发色团的β-紫罗酮环周围的构象发生了变化。[4]八元视紫红质的激光闪光光解表明，光致变色过程对应于发色团中反式C-C双键高度扭曲构象的弛豫途径<11><12>。[5]红外光谱表明，该席夫碱被质子化。[6]在细菌视紫红质的光循环中， ...更多信息 f之间的相互作用的蛋白质部分和质子化的席夫碱的视黄基发色团观察到在两个阶段的L和N的中间体通过FTIR光谱。[7]红外光谱分析表明，在细菌视紫红质的中间体N中，Asp-96发生了去质子化，而Asp-85发生了质子化。[8]发色团萃取实验表明，视黄色素的光漂白是由视黄色素中C-C双键的顺反异构化引发<11><12>的。[9]有人认为，metaiodopsin II的寿命将远远短于metarhodopsin II。[10]目前正在研究对碘视蛋白特异性的我们的单克隆抗体的表位的分析。碘视蛋白的C端可能是抗原位点。[11]利用纯化的牛视紫红质激酶将碘视蛋白的光漂白中间体磷酸化。[12]从鸡视网膜中部分纯化了一种推定的视锥细胞特异性G蛋白α亚基的候选蛋白。[13]根据碘视蛋白的互补DNA推导出其氨基酸序列。克隆鸡视网膜中其他锥状色素的工作正在进行中。少