权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

RELATIONS BETWEEN FLOW STRUCTURE AND VASCULAR PATHOPHYSIOLOGICAL CHANGES IN THE CARDIOVASCULAR SYSTEM --STUDIED BY ULTRASONIC DIGITAL FLOW IMAGING SYSTEM--

心血管系统血流结构与血管病理生理变化之间的关系——超声数字血流成像系统研究——

基本信息

批准号：
59440043
负责人：
INOUE Michitoshi
金额：
$ 19.2万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1985
项目状态：
已结题

项目摘要

Specific flow dynamics would play a significant role in the initiation and development of atherosclerosis. To elucidate the underlying mechanism of the atherogenesis with regard to local flow dynamics, we assessed the distribution of the wall shear stress in canine arteries by the high frequency multigate Doppler flowmetry, which allowed us to measure the instantaneous flow velocity profile in in vivo vessels. Then, we investigated the quantitative relation of the wall shear stress with the amount of the microfilament bundle in the endothelial cell as assessed by ultrastructual morphometry. After the left thoracotomy, the Doppler transducer was placed on the left anterior descending coronary artery (LAD), subclavian artery (SA), and thoracic descending aorta (DA) at the fixed angle (60 degrees), and the recording of flow velocity profile was performed in 5 dogs. The wall shear stress was determined as a product of the velocity gradient in the boudary layer and the kinematic viscocity o … More f the blood. After the Doppler examination, the studied vessels were perfusion-fixed under the constant pressure (100 mmHg), and the ultrastructural morphometric analysis was performed longitudinally and transversely in the length of 0.3mm in x17720 photos. The data are summerized in the following tables 1 and 2.Peak wall shear stress was signigicantly lower in LAD compared with those in SA and DA. However, the F/C ratio, the ratio of the area of microfilament bundle to the area of endothelial cell, was significantly greater in LAD than those in SA and DA. Thus, these results suggest that in physiological conditions the amount of the microfilament bundle in the endothelial cell, which is compatible with actin filament stress fiber, seems not to be determined only by the wall shear stress. The cytoskeletal structure of the coronary arterial endothelial cell is considered to be quite specific when compared with that in other artery, and this finding seems to be helpful in assessing the pathology of the coronay artery. Less

特定的血流动力学在动脉粥样硬化的发生和发展中起着重要作用。为了阐明局部血流动力学方面的动脉粥样硬化形成的潜在机制，我们评估了在犬动脉壁切应力的分布，通过高频多门多普勒血流仪，这使我们能够测量瞬时流速分布在体内血管。然后，我们研究了壁面切应力与内皮细胞中微丝束数量的定量关系，如通过超微结构形态计量学评估的。在5只犬左胸切口后，将多普勒探头以固定角度（60 °）分别置于左冠状动脉前降支（LAD）、锁骨下动脉（SA）和胸降主动脉（DA），记录血流速度分布。壁面剪应力是边界层中速度梯度与边界层运动粘度的乘积， ...更多信息 F血液。多普勒检查后，将所研究的血管在恒定压力（100 mmHg）下灌注固定，并在x17720张照片中进行0.3mm长的纵向和横向超微结构形态计量学分析。结果如下表1和表2所示。LAD的峰值壁切应力显著低于SA和DA。然而，LAD的F/C比值（微丝束面积与内皮细胞面积的比值）显著高于SA和DA。因此，这些结果表明，在生理条件下，内皮细胞中与肌动蛋白丝应力纤维相容的微丝束的量，似乎不仅仅由壁面剪应力决定。冠状动脉内皮细胞的细胞骨架结构与其他动脉相比具有很强的特异性，这一发现有助于评价冠状动脉的病理变化。少