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Research for the establishment of combined modality therapy including targeting chemotherapy for cancer of the pancreas.

研究建立联合疗法，包括针对胰腺癌的靶向化疗。

基本信息

批准号：
59440056
负责人：
SATO Toshio
金额：
$ 10.56万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1986
项目状态：
已结题

项目摘要

1. Twelve cultured human pancreatic cancer cell lines were established and it was demonstrated that they were good model of human pancreatic duct cell cancer.2. Anti-tumor chemo and radio sensitivity test of pancreatic cancer1) Pancreatic cancer cell lines showed good responses to anti-tumor drugs at clinically achievable concentration2) Pancreatic cancer, though resistant to irradiation, to irradiation at high irradiation dose level with the use of hypoxic cell sensitizer showed elevated response PK-23.3. Monoclonal antibody F-30 which specifically reacted with human pancreatic cancer was produced. Pancreatic cancer associated antigen detected by McAb F-30 was cell membrane integrated protein with molecular weight of 190,000 dalton.4. Analysis of <^(131)I> -labeled antibody F30 to detect tumor localization was performed.5. Human derives anti-human pancreatic cancer antibody was obtained using EB virus transformed B cell lines established from regional lymph node cells. But this B cell line was unstable about their ability to secrete antibody.6. Pancreatic cancer call clustes were detected in cytology from perfusate of gastroduodenal artery in resected specimen.7. Anti-tumor chemoshsitivity test was performed using ADM encapsulated in liposome and it was shown that encapsulated ADM was less effective than free ADN.

1．建立了12个培养的人胰腺癌细胞株，并证明其是良好的人胰管细胞癌模型。 2．胰腺癌的抗肿瘤化疗和放射敏感性试验1) 胰腺癌细胞系对临床可达到浓度的抗肿瘤药物表现出良好的反应2) 胰腺癌虽然对照射有抵抗力，但在使用缺氧细胞增敏剂的高照射剂量水平下，显示出较高的反应PK-23.3。生产出与人胰腺癌特异性反应的单克隆抗体F-30。单克隆抗体F-30检测到的胰腺癌相关抗原为细胞膜整合蛋白，分子量为190,000道尔顿。4．进行<^(131)I>-标记抗体F30的分析以检测肿瘤定位。5．使用从区域淋巴结细胞建立的 EB 病毒转化的 B 细胞系获得人源抗人胰腺癌抗体。但该B细胞系分泌抗体的能力不稳定。6．切除标本胃十二指肠动脉灌注液细胞学检测发现胰腺癌细胞簇。 7．使用脂质体封装的ADM进行抗肿瘤化学活性测试，结果表明封装的ADM不如游离的ADN有效。