The interrelationship between each gut organ modulated by gastrointestinal hormones

我们的研究包括以下几个方面：(1)建立犬慢性胃瘘模型，使我们能够在清醒状态下持续收集胰液和胃液。(2)建立对胰腺外分泌和胆囊收缩起重要生理作用的血浆胆囊素(CCK)的放射免疫分析方法。(3)胃泌素释放肽(GRP)、神经肽C、YY肽(PYY)、组氨酸异亮氨酸(PHI)等胃肠激素的作用研究。(4)对十二指肠内注入脂肪或食物刺激后慢性胃瘘犬胰腺外分泌和内源性释放CCK进行同步检测，以确定CCK是否具有真正的生理意义。(5)观察人体内源性释放CCK和胃肠道器官切除后胃肠激素释放的变化。(6)胃肠激素产生细胞包括CCK免疫组织化学定位与胃肠激素组织浓度放射免疫测定的对比研究。

Our research project consists of the following categories.(1) Construction of the experimental model of the dog with chronic and gastric fistulas which allows us continuous collection of pancreatic and gastric juice in the conscious state.(2) Establishment of the specific and sensitive radioimmunoassay for plasma cholecystokinin (CCK) that plays a physiologically important role for exocrine pancreatic secretion and gallbladder contraction.(3) Study on the effect of gastrointestinal hormones, such as Gastrin Releasing Peptide (GRP), Neuromedin C, Peptide YY (PYY), Peptide Histidine Isoleucine (PHI), on exocrine pancreatic secretion and plasma levels of gastrointestinal hormones including CCK to clarify the biological activity and physiological importance of these peptides.(4) Simultaneous measurment of exocrine pancreatic secretion and endogeneouslly released CCK on dogs with chronic and gastric fistulas following introduodenal instillation of fat or food stimulation to determine if CCK has true physiological importance.(5) Observation on the endogeneouslly released CCK in human and on the changes of the release of gastrointestinal hormones following resection of gut organs in the digestive surgery.(6) Comparative study of the immunohistochemical localization of the gastrointestinal hormone producing cells including CCK and radioimmunoassay measurement of tissue concentrations of gastrointestinal hormones.