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The interrelationship between each gut organ modulated by gastrointestinal hormones

胃肠激素调节的各肠道器官之间的相互关系

基本信息

批准号：
59440057
负责人：
TOBE Takayoshi
金额：
$ 8.96万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1985
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59440057/
关键词：
dog with chronic pancreatic and gastric fistulas gastrointestinal hormones CCK radioimmunoassay exocrine pancreatic secretion GRP NeuromedinC PYY

项目摘要

Our research project consists of the following categories.(1) Construction of the experimental model of the dog with chronic and gastric fistulas which allows us continuous collection of pancreatic and gastric juice in the conscious state.(2) Establishment of the specific and sensitive radioimmunoassay for plasma cholecystokinin (CCK) that plays a physiologically important role for exocrine pancreatic secretion and gallbladder contraction.(3) Study on the effect of gastrointestinal hormones, such as Gastrin Releasing Peptide (GRP), Neuromedin C, Peptide YY (PYY), Peptide Histidine Isoleucine (PHI), on exocrine pancreatic secretion and plasma levels of gastrointestinal hormones including CCK to clarify the biological activity and physiological importance of these peptides.(4) Simultaneous measurment of exocrine pancreatic secretion and endogeneouslly released CCK on dogs with chronic and gastric fistulas following introduodenal instillation of fat or food stimulation to determine if CCK has true physiological importance.(5) Observation on the endogeneouslly released CCK in human and on the changes of the release of gastrointestinal hormones following resection of gut organs in the digestive surgery.(6) Comparative study of the immunohistochemical localization of the gastrointestinal hormone producing cells including CCK and radioimmunoassay measurement of tissue concentrations of gastrointestinal hormones.

我们的研究项目包括以下几类。(1)建立慢性胃瘘犬的实验模型，使我们能够在意识状态下连续采集胰胃液。(2)建立特异性、敏感性的血浆胆囊收缩素（CCK）放射免疫测定方法，CCK在外分泌胰腺和胆囊收缩中起重要的生理作用。(3)研究胃肠激素如胃泌素释放肽（GRP）、神经素C （Neuromedin C）、多肽YY （PYY）、多肽组氨酸异亮氨酸（PHI）等对胰腺外分泌和血浆CCK等胃肠激素水平的影响，阐明这些肽的生物活性和生理意义。(4)同时测量慢性和胃瘘犬在十二指肠内注入脂肪或食物刺激后的外分泌胰腺分泌和内源性释放的CCK，以确定CCK是否具有真正的生理意义。(5)人体内内源性CCK的释放及消化道手术切除肠道器官后胃肠道激素释放变化的观察。(6)胃肠激素产生细胞（包括CCK）的免疫组化定位与胃肠激素组织浓度的放射免疫测定的比较研究。