An in vitro Method for Testing the Sensitivity to Drugs of Human Malignant Bone and Soft Tissue Tumor

人恶性骨与软组织肿瘤药物敏感性的体外检测方法

• 批准号: 59440063
• 负责人: ISHII SEIICHI
• 金额: $ 4.8万
• 依托单位: SAPPORO MEDICAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59440063/
• 关键词: HUMAN RHABDOMYOSARCOMA; OSTEOSARCOMA; NUDE MOUSE; TISSUE CULTURE; AUTORADIOGRAPHIC STUDIES; 核酸標識化合物; 核酸合成抑制効果

Sensitivity test to anticancer drugs by mesuring the supression of nucleic acid synthesis is considered to be useful in clinical application. We have developed new test system in which sensitivity is investigated with cultured cells from transplanted tumors of original sarcomas onto the back of nude mice. Results are as follows.(1) We examined sensitivity of anticancer drugs in 7 human rhabdomyosarcomas and 12 human osteosarcomas. The results of sensitivity to drugs were obtained in about 70 percent of all of the trials made for our sarcoma cases.(2) Rhabdomyosarcomas showed a tendency to be sensitive to VCR, MMC, ADM, BLM MTX, ADM, MMC, CPM, ACT-D are estimated as high sensitive drugs to osteosarcomas.(3) We examined the reliability of the sensitivity test using <^3H> -UdR, <^3H> -TdR and <^(14)C> -formate. Triciated UdR was considered to be the most appropriate labeled precursor that can show accurately the degree of supression of nucleic acid synthesis.(4) The results of sensitivity … More were somewhat comparable with those achieved by mesuring cell survival. The drugs inhibited the regrowth of cultured cells from rhabdomyosarcomas and osteosarcomas completely, when the ratio of supression of nucleic acid synthesis caused by these drugs showed more than 75 percent.(5) The results of sensitivity to anticancer drugs were also comparable with those achieved by mesuring the success ratio of back transplantation of rhabdomyosarcomas, and of lung metastasis of osteosarcoma onto nude mice after treatment with drugs which showed sensitive in our test system.(6) We investigated whether the results of sensitivity showed the reproducibility or not, by repeating the sensitivity test 2 to 7 times over during 2 months for each osteosarcoma case. 6 out of 8 osteosarcomas showed same sensitivity pattern to anticancer drugs in each test we repeated.(7) The correlation between sensitivity testing and clinical results was examined, showing true positive rate: 50 percent and true negative rate: 66.6 percent, when tested by mesuring the supression of nucleic acid synthesis. We concluded our sensitivity test has a significance when we want to know non-effective anticancer drug in clinical use. Less

通过测量核酸合成的抑制来进行抗癌药物的敏感性测试被认为在临床应用中是有用的。我们开发了新的测试系统，用来自原始肉瘤移植瘤到裸鼠背部的培养细胞来研究敏感性。结果如下。(1)我们检测了7例人类横纹肌肉瘤和12例人类骨肉瘤的抗癌药物敏感性。在我们对肉瘤病例进行的所有试验中，约70%获得了药物敏感性结果。(2)横纹肌肉瘤表现出对VCR、MMC、ADM、BLM敏感的倾向。MTX、ADM、MMC、CPM、ACT-D被估计为对骨肉瘤的高敏感性药物。(3)我们使用<^3H>检查了敏感性测试的可靠性。 -UdR、<^3H>-TdR 和 <^(14)C>-甲酸盐。 Triciated UdR被认为是最合适的标记前体，可以准确地显示核酸合成的抑制程度。(4)灵敏度结果在某种程度上与测量细胞存活率所获得的结果相当。当这些药物引起的核酸合成抑制率超过75％时，这些药物完全抑制了横纹肌肉瘤和骨肉瘤培养细胞的再生。（5）抗癌药物敏感性结果也与测量横纹肌肉瘤回移植和肺转移成功率的结果相当。 (6)我们通过对每个骨肉瘤病例在2个月内重复敏感性试验2至7次来调查敏感性结果是否显示出可重复性。在我们重复的每次测试中，8种骨肉瘤中有6种对抗癌药物表现出相同的敏感性模式。(7)检查敏感性测试与临床结果之间的相关性，通过测量核酸合成的抑制进行测试，显示真阳性率：50%，真阴性率：66.6%。我们得出结论，当我们想了解临床使用的无效抗癌药物时，我们的敏感性测试具有重要意义。较少的

项目成果

病態生理. 5-3. (1986)

病理生理学。5-3。

北海道整形災害外科学会誌. 29-1,2合伴. (1985)

北海道整形外科学会杂志 29-1，2 联合版（1985 年）。

SEIICHI, ISHII: "An in vitro Assay Procedure to Test Chemotherapeutic Drugs on Cells from Human Malignand Bone and Soft Tissue Tumors" BYOTAISEIRI. 5. 229-232 (1968)

SEIICHI、ISHII：“在人恶性肿瘤骨和软组织肿瘤细胞上测试化疗药物的体外测定程序”BYOTAISEIRI。

石井清一: 整形外科MOOK. 42. 187-195 (1986)

石井诚一：骨科 MOOK。42. 187-195 (1986)

SEIICHI, ISHII: "An in vitro Method for Testing the Sensitivity to Drugs of Human Malignant Bone and Soft Tissue Tumor" SEIKEIGEKA MOOK. 42. 187-195 (1986)

SEIICHI、ISHII：“一种体外测试人类恶性骨和软组织肿瘤药物敏感性的方法”SEIKEIGEKA MOOK。