Functional aspects of high endothelial venules (HEV) in experimental autoimmune-uveitis (EAU)

实验性自身免疫性葡萄膜炎 (EAU) 中高内皮微静脉 (HEV) 的功能方面

• 批准号: 5205446
• 负责人: Professor Dr. Manfred Zierhut
• 金额: --
• 依托单位: Institute of Medical Sciences
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 1999
• 资助国家: 德国
• 起止时间: 1998-12-31 至 2002-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5205446?language=en
• 关键词: Functional; aspects; endothelial; venules; HEV

Aims of the project: Characterization of HEV (high endothelial venules), cell adhesion molecule, cytokine and chemokine expression kinetics responsable for the HEV activation/inactivation and immunocompetent cell infiltrations in the early period and in the recurrence period of EAU (experimental autoimmune uveitis). This includes the evaluation of Interferon gamma (IFN-g) for HEV activation in IFN-g knock out mice. Modification of the expression kinetics by the HEV specific mAb MECA 325, the interferons a und ß (IFN-a/IFN-ß), the Tumor Necrosis Factor alpha (TNFa) inhibitor Rolipram and cytokine receptors (TNFa-RI, TNFa-RII, R-LT-ß (Lymphotoxin-betaReceptor). While the expression kinetics are analysed using immunocytochemistery and in situ hybridization, in vivo studies are intended to correlate these data with the cell traffic in the eye using scanning laser ophthalmoscopy (SLO).The scientific program is subject for two application periods of totally six years.

项目目的：表征HEV（高内皮小静脉），细胞粘附分子，细胞因子和趋化因子表达动力学，负责HEV激活/失活和EAU（实验性自身免疫性葡萄膜炎）早期和复发期的免疫活性细胞浸润。这包括干扰素γ (IFN-g)在IFN-g敲除小鼠中对HEV激活的评估。通过HEV特异性单抗meca325、干扰素a和β (IFN-a/IFN- β)、肿瘤坏死因子α (TNFa)抑制剂罗利普仑和细胞因子受体（TNFa- ri、TNFa- rii、R-LT- β（淋巴素β受体））修饰表达动力学。虽然使用免疫细胞化学和原位杂交分析了表达动力学，但体内研究旨在使用扫描激光检眼镜（SLO）将这些数据与眼睛中的细胞流量联系起来。科学项目有两个申请期，共六年。