Role of hemolymoh on metabolism, immune systems and endocrine systems in insects.

血溶素对昆虫代谢、免疫系统和内分泌系统的作用。

• 批准号: 60304009
• 负责人: OHTAKI Tetsuya
• 金额: $ 4.1万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60304009/
• 关键词: Vitellin; Vitellogenin; Ecdysone; JH; Egg-specific-protein; Sarcotoxin; Prophenoloxidase; "30K Protein"

Biosynthesis, metabolism, accumulation into particular tissues and physiological action of hemolymph protein, including vitellogenin, eggspecific protein, prophenoloxydase and bacteriocidal proteins, were studied. Action of insect hormones on the synthesis and activation of these proteins, and regulation of hormonal titer through hemolymph were also studied. In this research project, DNA sequences of a bacteriocidal protein in the fleshlfy and 30 KDa protein in silkworm gene were extensively studied. Fate of egg-specific protein in developing embryo of the silkworm was clarified. Role of ecdysone and other steroids on vitellogenesis was studied and suggestive data were obtained on the control mechanisms of ecdysone titer through hemolymph.I was found also that some kind of receptors of proteoglycan was involved in activation of prophenoloxidase which might play an important role on immune systems in insects.

研究了血淋巴蛋白质的生物合成、代谢、在特定组织中的积累和生理作用，包括卵黄蛋白原、卵特异蛋白、酚氧化酶原和杀菌蛋白。本文还研究了昆虫激素对这些蛋白质合成和活化的作用，以及通过血淋巴调节激素滴度。本研究对家蚕基因中的一个杀菌蛋白和一个30KDa蛋白的DNA序列进行了深入的研究。阐明了卵特异蛋白在家蚕胚胎发育过程中的去向。本文研究了蜕皮激素等激素对卵黄发生的影响，初步探讨了血淋巴调节蜕皮激素滴度的机制，并发现蛋白多糖的某些受体参与了酚氧化酶原的激活，而酚氧化酶原可能在昆虫免疫系统中起重要作用。

项目成果

J.Zhu;L.S.Indrasith;O.Yamashita: Biochimica et Biophysica Acta. 882. 427-436 (1986)

J.Zhu；L.S.Indrasith；O.Yamashita：生物化学与生物物理学学报。

S.Takahashi: Insect Biochemistry. 17. 141-152 (1987)

S.Takahashi：昆虫生物化学。

Y.Chinzei;H.Minoura: Medical 22 Veterinary Entomolgy. (1987)

Y.Chinzei；H.Minoura：医学 22 兽医昆虫学。

E. Ohnishi: "Growth and maturation of ovaries in isolated abdomens of Bombyx mori: Responses to ecdysteroids and othea steroids" Zoological Science. 4. (1987)

E. Ohnishi：“家蚕离体腹部卵巢的生长和成熟：对蜕皮类固醇和奥西类固醇的反应”动物学科学。

S. Izumi, K. Yamazaki, S. Tomino and H. Chino: "Biosynthesis of apolipophorin <III> by the fatbody in locust" Journal of Lipid Research. (1987)

S. Izumi、K. Yamazaki、S. Tomino 和 H. Chino：“蝗虫脂肪体生物合成载脂蛋白 <III>”《脂质研究杂志》。