Studies on vitellogenin and its receptor in salmonid using interactome techniques

当鱼生长到卵巢时，卵黄前体卵黄蛋白原(VG)与卵巢VG受体(VGR)结合后从血液进入卵母细胞。本研究首次利用切割相互作用组学技术鉴定了鲑鱼VG(SVG)上VGR结合区的一部分。在此基础上，克隆了一个新的C型VG(VGC)，并合成了限量的重组蛋白，为发现未发现的VGC受体奠定了基础。

When fish grows ovaries, a yolk precursor vitellogenin (Vg) is incorporated from the blood into oocyte following its binding to ovarian Vg receptor (VgR). This study first identified a portion of VgR-binding region on salmon Vg (sVg) using cutting-edged interactome techniques. Further, a novel C-type Vg (VgC) was cloned and its recombinant protein was synthesized with a limited amount; this provided a basis for discovering the unrevealed VgC receptor.