Studies on the Drug-Receptor Mechanisms of Chemical Transmitters and Autacoids

本课题组的研究涉及各种激动剂的剂量-反应曲线与受体结合的关系、受体亚型的分类、激动剂的脱敏机制以及疾病动物的受体功能。1.ACh受体的研究：川岛明确了豚鼠回肠上的M_2受体。渡边表现出ACh引起的虹膜平滑肌松弛的释放。Takayanagi通过药效和受体结合来显示激动剂活性的变化。吉田估计了M_2和M_2受体的3个结合部分。Gomi分析激动剂和钙的活性。Utida揭示了ACh对单个平滑肌细胞脱敏的机制。木村证明了糖尿病小鼠ACh受体的变化。2.血管上的受体机制：Tomita通过K电导证实了&lt；α&gt；和&lt；β&gt；受体的抑制机制。户田阐明了人类和猴子冠状动脉上组胺受体的亚型。石田展示了兔基底动脉上的嘌呤能受体亚型。Kasuya证明，Diabate大鼠肾上腺素活性增强的因素之一是内皮介导的血管扩张。3.类耳石的受体机制：栗山对豚鼠肺动脉上的组胺亚型进行了分类。久保田证实了豚鼠胆囊壁上的缩胆囊素受体。Fukuda估计了培养的鸡脊髓神经元上GABA受体与氯通道的关系。

The studies of our team deal with the relationship between dose-response curves and receptor bindings for variou agonists, the classification of receptor-subtypes, the desensitization mechanisms for agonists and the receptor functons in the disease animals. 1. Studies on ACh receptors: Kawashima clarified the <M_2> -receptor on the guinea-pig ileum. Watanabe showed the release of relaxing subustnces from iris smooth muscle induced by ACh. Takayanagi showed the changes of agonist activities by efficacy and receptor binding. Yoshida estimated the 3 binding portions of <M_2> -muscarinic receptor. Gomi analyzed the activity of agonists and Ca. Utida showed the mechanisms of ACh desensitization on the single smooth muscle cells. Kimura demonstrated the change of ACh receptors in the diabete mice. 2. The receptor mechanisms on the blood vessels: Tomita demonstrated the inhibitoy mechanisms of <alpha> and <beta> receptors by K conductance. Toda clarified the subtypes of histamine receptors on the coronary arteies from man and monkey. Ishida demonstrated the subtype of purinergic receptors on rabbit basilar artery. Kasuya showed that one of factors in the augmentation of adrenaline activity of the diabate rats was due to the endothelium-mediated vasodilation. 3. Receptor mechnisms of autacoids: Kuriyama classified the subtypes of histamie on the lung artery from guinea-pig. Kubota demonstrated the receptor of cholecystokinin on the ginea-pig gall bladder. Fukuda estimated the relationship between the GABA receptor and Cl channel on the cultured chick spinal cord neurons.