Studies on the Drug-Receptor Mechanisms of Chemical Transmitters and Autacoids

化学递质和自体酸的药物受体机制研究

• 批准号: 60304080
• 负责人: ISHIDA Yukio
• 金额: $ 5.5万
• 依托单位: The University of Tokushima, Faculty of Pharmaceutical Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60304080/
• 关键词: Chemical transmitter; Autacoid; Receptor; Desensitization; Acetylchline; Adrenaline; Histamine

The studies of our team deal with the relationship between dose-response curves and receptor bindings for variou agonists, the classification of receptor-subtypes, the desensitization mechanisms for agonists and the receptor functons in the disease animals. 1. Studies on ACh receptors: Kawashima clarified the <M_2> -receptor on the guinea-pig ileum. Watanabe showed the release of relaxing subustnces from iris smooth muscle induced by ACh. Takayanagi showed the changes of agonist activities by efficacy and receptor binding. Yoshida estimated the 3 binding portions of <M_2> -muscarinic receptor. Gomi analyzed the activity of agonists and Ca. Utida showed the mechanisms of ACh desensitization on the single smooth muscle cells. Kimura demonstrated the change of ACh receptors in the diabete mice. 2. The receptor mechanisms on the blood vessels: Tomita demonstrated the inhibitoy mechanisms of <alpha> and <beta> receptors by K conductance. Toda clarified the subtypes of histamine receptors on the coronary arteies from man and monkey. Ishida demonstrated the subtype of purinergic receptors on rabbit basilar artery. Kasuya showed that one of factors in the augmentation of adrenaline activity of the diabate rats was due to the endothelium-mediated vasodilation. 3. Receptor mechnisms of autacoids: Kuriyama classified the subtypes of histamie on the lung artery from guinea-pig. Kubota demonstrated the receptor of cholecystokinin on the ginea-pig gall bladder. Fukuda estimated the relationship between the GABA receptor and Cl channel on the cultured chick spinal cord neurons.

本课题组的研究内容包括：各种激动剂的剂量-反应曲线与受体结合的关系、受体亚型的分类、激动剂的脱敏机制以及受体在疾病动物体内的功能。1.乙酰胆碱受体的研究：Kawashima阐明了<M_2>豚鼠回肠上的乙酰胆碱受体。Watanabe显示ACh可引起虹膜平滑肌释放舒张性亚单位。Takayanagi通过药效和受体结合显示了激动剂活性的变化。Yoshida估计了β-毒蕈碱受体的3个结合部位<M_2>。Gomi分析了激动剂和Ca. Utida显示了ACh对单个平滑肌细胞的脱敏机制。Kimura等证实了糖尿病小鼠ACh受体的变化。2.血管上的受体机制：Tomita通过K电导证明了受体的作用机制<alpha><beta>。户田阐明了人和猴冠状动脉组胺受体的亚型。Ishida证实了家兔基底动脉上嘌呤能受体的亚型。Kasuya的研究表明，糖尿病大鼠肾上腺素活性增强的因素之一是由于内皮介导的血管舒张。3.自育激素受体机制：Kuriyama等对豚鼠肺动脉组织组胺进行了亚型分类。久保田证明胆囊收缩素受体在豚鼠胆囊上。Fukuda在体外培养的鸡脊髓神经元上研究了GABA受体与Cl通道的关系。