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Regulation of axoplasmic transport

轴浆运输的调节

基本信息

批准号：
60440026
负责人：
KUROKAWA Masanori
金额：
$ 19.65万
依托单位：
Institute of Brain Research, Tokyo University Faculty of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

1) Flow pattern of cytoskeletal proteins in axons was analysed by SDS-polyacrylamide gel electrophoresis coupled with fluorography, after labelling the art L5 ganglion with L-[^<35>S] methionine. A portion of tubulin migrated at 3.2 mm/day; this portion contrained most of intra-axonal tau factors, and was depolymerised by colchicine and at low temperature. The remaining portion of tubulin migrated at 1.8 mm/day, contained less tau factors, and comparatively resistant to colchicine and low temperature. This portion is considered to serve as a nucleus in polymer isation of ihtra-axonal tubulin in situ.2) GD1<alpha>, a ganglioside species in Xenopus sciatic nerve, was found to migrate at a slow rate i.e. at around 1 mm/day at 15゜C, in sharp contrast to rapid transport of gangliosides so far reported in various animal species. Heterogeneity of ganglioside transport was thus indicated. Further, transport of CMP-sialic acid was found, suggesting the possibility of additional sialosylation of the ganglioside in axon terminals.3) Co-existence of choline acetyltransferase and glutamic acid decarboxylase was found in the anterior horn cells in various species by using ultramicroassay techniques newly. developed in this laboratory. This raises the possibility of <gamma>-aminobutyric acid to play a role other than a naeurotransmitter in this purely cholinergic neurone.

1)在用L-[^ S]蛋氨酸标记art L5神经节后，通过SDS-聚丙烯酰胺凝胶电泳结合荧光照相术分析轴突中细胞骨架蛋白的流动模式<35>。一部分微管蛋白以3.2 mm/天的速度迁移;这部分抑制了大多数轴突内tau因子，并在低温下被秋水仙素解聚。微管蛋白的其余部分以1.8 mm/天的速度迁移，含有较少的tau因子，并且对秋水仙素和低温具有相对抗性。该部分被认为是作为轴突内微管蛋白原位聚合的核。2）发现GD 1<alpha>（爪蟾坐骨神经中的神经节苷脂种类）以缓慢的速率迁移，即在15 ℃下以约1 mm/天迁移，与迄今为止在各种动物物种中报道的神经节苷脂的快速运输形成鲜明对比。因此，神经节苷脂转运的异质性。此外，还发现了CMP-唾液酸的转运，提示神经节苷脂在轴突终末可能存在额外的唾液糖基化作用。3）超微结构分析发现，胆碱乙酰转移酶和谷氨酸脱羧酶在不同物种的前角细胞中共存。在这个实验室里开发的。这增加了<gamma>-氨基丁酸在这个纯粹的胆碱能神经元中发挥神经递质以外的作用的可能性。