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Histo- ＆ cytochemical studies on the membrane biogenesis and recycling

膜生物发生和回收的组织和细胞化学研究

基本信息

批准号：
60440023
负责人：
HIRANO Hiroshi
金额：
$ 19.58万
依托单位：
Kyorin University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1988
项目状态：
已结题

项目摘要

Membrane biogenesis and recycling processes were studied in various organs and tissues principally based on the histo and cytochemical methods employing related specific antibodies and lectins as probes. Obtained results are followings: The notion of an insulin-dependent translocation of the glucose transporter in rat adipocytes was confirmed by immunoblotting and reconstitution of glucose transport activity of subcellular fractions, and also explored by cytochemical techniques. Cyto-chemically the glucose transporter was distributed in rat renal medulla, particularly localizing in the baso-lateral surface membranes of the collecting ducts, the thick portion of Henle and S3 of the proximal portion. In the activated parietal cells of human gastric mucosa, most of the dba-negative tubulovesicles in the cytoplasm were rapidly fused with the canaliculi membrane which was intensely stained with labeled-DBA. Changes in DBA-staining was quantitatively measured by light microscopy. In human pl … More atelets, the membrane of the open canalicular system (OCS) was different from the cell membrane in glycoconjugate constituents. when treated with cationized ferritin (CF), the OCS lost its openings at the cell surface, and concentrated in the cell interior forming conglomerates of tubular and vacuolar structures, in which endocytosed CF particles were contained and microfilaments were involved. When activated with ADP and/or Teleocidin, the limiting membrane of granules was fused with the membrane of the OCS and occasionally also with the cell membrane.In rat urinary bladder mucosa, the luminal surface membrane of the superficial cell revealed characteristic changes in lectin- and CF-binding pattern in parallel with the structural changes during embryogenesis and carcinogenesis. In chick embryonic skin, EGF (epidermal growth factor) as well as vitamin A induced in vitro striking changes in the basement membrane structure and also in distribution of EGF itself within the epidermis, which were accompanied by prevention of keratinization process. Cytochemical properties of the changing basement membrane were examined. The morphogenesis of the basement membrane was explored in the so-called recombination experiments using isolated embryonic epidermis and dermis as starting materials. Less

以组织化学和细胞化学方法为基础，以相关的特异性抗体和凝集素为探针，研究了各种器官和组织的膜生物发生和再循环过程。获得的结果如下：通过免疫印迹和亚细胞组分葡萄糖转运活性的重建证实了大鼠脂肪细胞中葡萄糖转运蛋白的胰岛素依赖性易位的概念，并通过细胞化学技术进行了探索。在细胞化学上，葡萄糖转运蛋白分布于大鼠肾髓质，尤其集中于集合管的基底外表面膜、Henle管的厚部和近端部的S3。人胃粘膜壁细胞活化后，胞浆内大部分dba阴性的小管状泡与标记的DBA强染色的小管膜迅速融合。通过光学显微镜定量测量DBA染色的变化。在人体内， ...更多信息此外，开放小管系统（OCS）的膜与细胞膜的糖结合物成分不同。当用阳离子化的铁蛋白（CF）处理时，OCS失去其在细胞表面的开口，并集中在细胞内部形成管状和空泡结构的聚集体，其中包含内吞的CF颗粒并涉及微丝。当用ADP和/或Teleocidin激活时，颗粒的界膜与OCS的膜融合，偶尔也与细胞膜融合。在大鼠膀胱粘膜中，表面细胞的腔表面膜显示出与胚胎发生和癌变过程中的结构变化平行的凝集素和CF结合模式的特征性变化。在鸡胚皮肤中，EGF（表皮生长因子）以及维生素A在体外诱导基底膜结构以及表皮内EGF本身的分布的显著变化，这伴随着防止角化过程。改变基底膜的细胞化学性质进行了检查。在所谓的重组实验中，使用分离的胚胎表皮和真皮作为起始材料，探索了基底膜的形态发生。少