Histo- ＆ cytochemical studies on the membrane biogenesis and recycling

主要基于组织和细胞化学方法，利用相关特异性抗体和凝集素作为探针，研究了不同器官和组织中的膜生物发生和循环过程。获得的结果如下：通过免疫印迹和亚细胞部分葡萄糖转运活性的重建，证实了大鼠脂肪细胞中葡萄糖转运蛋白胰岛素依赖性易位的概念，并通过细胞化学技术进行了探索。细胞化学上葡萄糖转运体分布于大鼠肾髓质，特别是集中于集管基底外侧表面膜、Henle厚层和近端S3层。在活化的人胃粘膜壁细胞中，细胞质中大部分ba阴性的小管泡与小管膜迅速融合，小管膜被标记的dba强烈染色。光镜下定量测定dba染色的变化。在人类小细胞中，开放小管系统（OCS）的膜与细胞膜的糖缀合物成分不同。经阳离子化铁蛋白（CF）处理后，OCS在细胞表面失去开口，集中在细胞内部形成管状和液泡状结构的聚集体，其中含有内吞的CF颗粒，并涉及微丝。当被ADP和/或远杀虫素激活时，颗粒的限制膜与OCS膜融合，偶尔也与细胞膜融合。在大鼠膀胱粘膜中，浅表细胞的管腔表面膜在胚胎发生和癌变过程中显示出凝集素和cf结合模式的特征性变化。在鸡胚皮肤中，EGF（表皮生长因子）和维生素A在体外诱导基底膜结构和表皮内EGF本身的分布发生显著变化，并伴有角化过程的阻止。观察了基底膜变化的细胞化学性质。以离体胚胎表皮和真皮为起始材料，通过所谓的重组实验探讨了基底膜的形态发生。少

Membrane biogenesis and recycling processes were studied in various organs and tissues principally based on the histo and cytochemical methods employing related specific antibodies and lectins as probes. Obtained results are followings: The notion of an insulin-dependent translocation of the glucose transporter in rat adipocytes was confirmed by immunoblotting and reconstitution of glucose transport activity of subcellular fractions, and also explored by cytochemical techniques. Cyto-chemically the glucose transporter was distributed in rat renal medulla, particularly localizing in the baso-lateral surface membranes of the collecting ducts, the thick portion of Henle and S3 of the proximal portion. In the activated parietal cells of human gastric mucosa, most of the dba-negative tubulovesicles in the cytoplasm were rapidly fused with the canaliculi membrane which was intensely stained with labeled-DBA. Changes in DBA-staining was quantitatively measured by light microscopy. In human pl … More atelets, the membrane of the open canalicular system (OCS) was different from the cell membrane in glycoconjugate constituents. when treated with cationized ferritin (CF), the OCS lost its openings at the cell surface, and concentrated in the cell interior forming conglomerates of tubular and vacuolar structures, in which endocytosed CF particles were contained and microfilaments were involved. When activated with ADP and/or Teleocidin, the limiting membrane of granules was fused with the membrane of the OCS and occasionally also with the cell membrane.In rat urinary bladder mucosa, the luminal surface membrane of the superficial cell revealed characteristic changes in lectin- and CF-binding pattern in parallel with the structural changes during embryogenesis and carcinogenesis. In chick embryonic skin, EGF (epidermal growth factor) as well as vitamin A induced in vitro striking changes in the basement membrane structure and also in distribution of EGF itself within the epidermis, which were accompanied by prevention of keratinization process. Cytochemical properties of the changing basement membrane were examined. The morphogenesis of the basement membrane was explored in the so-called recombination experiments using isolated embryonic epidermis and dermis as starting materials. Less