Histo- & cytochemical study of endogenous lectin

历史-

• 批准号: 09470004
• 负责人: HIRANO Hiroshi
• 金额: $ 8.96万
• 依托单位: Kyorin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470004/
• 关键词: Endogenous IectinI; Exogenous Iectin; sugar residue; gIycosyI transferase; histo-& cytochemistry; imaging; metastasis; differentiation; ガレクチン; O-GlcNAc; 光顕観察; 電顕観察; 線虫

As is well known, lectins are carbohydrate-binding proteins or glycoproteins of non-immune origin. In histochemistry and cytochemistry, labeled lectins have been widely used as a probe for the detection of carbohydrates. With the use of exogenously administered lectins, changes in glycoconjugates during the course of development, aging malignant transformation and degeneration have been successfully visualized in situ. Visualization of lectin binding pattern can be achieved not only by conventional light and electron microscopy but also by employing confocal laser scanning microscope and a combination of high voltage transmission electron microscopy and backscattered scanning electron imaging.Recently, in addition to so-called "exogenous lectins" as above, much attention has been paid to "endogenous lectins", in particular, animal lectins, with special reference to their expression and also possible roles.In vertebrates, β-galactoside-binding lectins, galectins, have been isolated from … More several tissues of different animals. Chick embryos, for example, have at least two different kinds of galectins, the 14- and 16-kD lectins, which belong to the family of animal metal-independent lectins or S-type, and exist in differentiating chick skin. Both cDNA and genomic DNA for these galectins were cloned, and the nucleotide sequence as well as the complete amino acid sequence were determined. Immunohistochemically these two isolectins revealed changes in expression in the epidermis and dermis of chick embryonic tarsometatarsal skin during the course of development both in vivo and in vitro. Differences between the 14, and 16-kD lectins were obvious particularly in in vitro experiments such as in retinol-induced mucous metaplasia. Changes in expression of these isolectins were in parallel with those in in situ hybridization signals. Both 14- and 16-kD galectins should play an important role in differentiation of the skin, and the gene expression of these two lectins is regulated independently.We already examined the localization of an endogenous 14-kD galectin in normal humall skin, and the 16-kD and the 32-kD galectins in the developing nematode Caenorhabditis elegans. Besides, we investigated expression of macrophage galactos/N-acetylgalactosamin-specific C-type lectin in normal mouse tissues and found that its location varied at different stages of development as well.Endogenous animal lectins and their complementary sugars have been round to be involved in fundamental and key roles playing in cells and tissues. In combination with both of the exogenous and endogenous lectins, the lectin histo- and cytochemistry should contribute much in elucidation in situ of function and significance or glycoconjugates. Less

众所周知，凝集素是非免疫来源的碳水化合物结合蛋白或糖蛋白。在组织化学和细胞化学中，标记凝集素已被广泛用作检测碳水化合物的探针。随着外源性凝集素的应用，糖复合物在发育、衰老、恶性转化和变性过程中的变化已被成功地原位观察到。凝集素结合模式的可视化不仅可以通过传统的光学显微镜和电子显微镜实现，还可以通过共聚焦激光扫描显微镜以及高压透射电子显微镜和背散射扫描电子成像的结合来实现。最近，除了上述所谓的“外源性凝集素”之外，“内源性凝集素”，特别是动物凝集素，在脊椎动物中，β-半乳糖苷结合凝集素，半乳糖凝集素，已经从 ...更多信息 几种不同动物的组织例如，鸡胚具有至少两种不同种类的半乳糖凝集素，14-和16-kD凝集素，其属于动物金属非依赖性凝集素或S-型家族，并且存在于分化的鸡皮肤中。克隆了这些半乳糖凝集素的cDNA和基因组DNA，并测定了核苷酸序列和完整的氨基酸序列。免疫组化显示，这两个isolectins的表达在鸡胚跗跖皮肤的表皮和真皮在体内和体外的发展过程中的变化。14和16-kD凝集素之间的差异是明显的，特别是在体外实验，如在视黄醇诱导的粘液化生。这些异凝集素的表达变化与原位杂交信号的变化平行。14-kD和16-kD半乳糖凝集素在皮肤的分化过程中起重要作用，这两种凝集素的基因表达是独立调节的，我们已经研究了内源性14-kD半乳糖凝集素在正常人皮肤中的定位，以及16-kD和32-kD半乳糖凝集素在发育中的线虫中的定位。此外，我们还研究了巨噬细胞半乳糖/N-乙酰半乳糖胺特异性C型凝集素在正常小鼠组织中的表达，发现其在不同发育阶段的定位也不同，内源性动物凝集素及其互补糖已被认为参与了细胞和组织的基础和关键作用。结合外源性和内源性凝集素，凝集素的组织化学和细胞化学将有助于阐明糖缀合物的功能和意义。少

项目成果

Yamaguchi H, Osaki T, Taguchi H, Yamamoto T, Hanawa T, Fukuda M, Kawakami H, Hirano H, Kamiya S.: "Growth inhibition of Helicobacter pylori by monoclonal antibody to heat-shock protein 60"Microbiol Immunol.. 41. 909-916 (1997)

Yamaguchi H、Osaki T、Taguchi H、Yamamoto T、Hanawa T、Fukuda M、Kawakami H、Hirano H、Kamiya S.：“热休克蛋白 60 的单克隆抗体对幽门螺杆菌的生长抑制”微生物免疫学 41。

川上速人、平野寛: "血小板の電子顕微鏡 活性化と突起形成"MEDICO. 29. 12870-12871 (1998)

Hayato Kawakami、Hiroshi Hirano：“电子显微镜激活和血小板的突出形成”MEDICO 29. 12870-12871 (1998)。

Yamamoto K, Kawakami H, Sugiyama M, Atomi Y, Hirano H: "Immunohistochemical localization of β-1,4-galactosyltransferase in human pancreatic tissues"Pancras. 19. 137-142 (1999)

Yamamoto K、Kawakami H、Sugiyama M、Atomi Y、Hirano H：“人胰腺组织中 β-1,4-半乳糖基转移酶的免疫组织化学定位”Pancras。 19. 137-142 (1999)

Takata K, Hirano H, Kasahara M: "Transport of glucose across the blood-tissue barriers"Int Rev. Cytol 172. 1-53 (1997)

Takata K、Hirano H、Kasahara M：“葡萄糖穿过血液组织屏障的转运”Int Rev. Cytol 172. 1-53 (1997)

Mizuochi S, Akimoto Y, Imai Y,Hirano H, Irimura T:: "Unique tissue distributon of a mouse macrophage C-type lectin."GIycobioIogy. 7. 137-146 (1997)

Mizuochi S、Akimoto Y、Imai Y、Hirano H、Irimura T：：“小鼠巨噬细胞 C 型凝集素的独特组织分布。”糖生物学。