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Significance of bacterial pili on urinary tract infection and application of anti-pili antibody to the treatment

细菌菌毛对尿路感染的意义及抗菌毛抗体在治疗中的应用

基本信息

批准号：
60440076
负责人：
KUMAZAWA Joichi
金额：
$ 10.05万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1988
项目状态：
已结题

项目摘要

1. Distribution of bacterial pili: Frequency of piliated bacteria in clinical isolates from urinary tract infeciton (UTI) was examined. High frequency was observed in E. coli and Serratia sp. and low frequency was observed in P. aeruginosa and Proteus sp. Mannose resistant (MR) and mannose sensitive (MS) pili was observed in E. coli and Serratia sp.2. Cloning of pili-gene and host response of piliation: Cloned DNA was obtained from clinical isolate of S. marcescens and either MR or MS pili gene was transfered to non-piliated E.coli with gene manipulation technique. By the use of these new strain, MS piliated bacteria rather than MR pili stimulated more severely scar formation of the kidney. This scar formation was prophylacted by superoxide dismutase (SOD). This findings suggested that superoxide (SO) was produced by the stimulation of MS pili, and destructed surrounding renal tissue.3. Host defense mechanism on UTI: Polymorphornuclear leukocytes (PMNL) was main factor of host defense mechanisms against UTI. However, function of PMNL was suppressed by ligh osmolality in the urine of renal medulla. As the main component of high osmolality in them was NaCl and urea, influence of high concentration of NaCl and ures on PMNL function was investigated. Energy dependent and indepent mechanisms were existed in it.

1.细菌菌毛的分布：检查了尿路感染（UTI）临床分离株中菌毛的频率。在大肠杆菌和沙雷氏菌属中观察到高频率。在铜绿假单胞菌和变形杆菌中观察到频率较低。在大肠杆菌和沙雷氏菌 sp.2 中观察到甘露糖抗性 (MR) 和甘露糖敏感 (MS) 菌毛。菌毛基因的克隆和宿主对毛发的反应：从粘质链球菌的临床分离物中获得克隆的DNA，并通过基因操作技术将MR或MS菌毛基因转移至非毛发状大肠杆菌中。通过使用这些新菌株，MS 菌毛细菌（而不是 MR 菌毛细菌）更严重地刺激了肾脏的疤痕形成。超氧化物歧化酶（SOD）可以预防这种疤痕的形成。研究结果表明，MS菌毛受刺激后产生超氧化物(SO)，并破坏周围肾组织。3．宿主对UTI的防御机制：多形核白细胞（PMNL）是宿主针对UTI的防御机制的主要因素。然而，肾髓质尿液中的低渗透压会抑制 PMNL 的功能。由于其高渗透压的主要成分是氯化钠和尿素，因此研究了高浓度的氯化钠和尿素对PMNL功能的影响。其中存在能量依赖机制和独立机制。