Analysis of bacterial and host factors in urinary tract infection

尿路感染细菌及宿主因素分析

• 批准号: 05404054
• 负责人: KUMAZAWA Joichi
• 金额: $ 17.92万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05404054/
• 关键词: UTI; fimbriae; adhesin; Active oxygen species; PMN; antioxidant; Escherichia coli; Pseudomonas aerginosa; 活性酸素; 腎盂腎炎; バイオフィルム; 上行性感染; 膀胱異物; 抗菌剤; 抗炎症剤; 多核白血球

We investigated bacterial and host factors in urinary tract infection (UTI). Bacteria isolated from UTI has various kinds of fimbriae on their surface to adhere to urinary mucosa. Adhesin located on the tip of fimbriae is found to stimulate polymorphonuclear leukocytes to release large amount of active oxygen species, which is toxic to surrounding renal tissue, resulting in renal scarring. Prevention of renal scar formation is one of the most important thing in the treatment of renal infection. We found that some kinds of antioxidants such as Ebselen, ulinastatin and steroid had preventive effect on renal scarring, and that these substances were to use combined with antimicrobial agents in the treatment of renal infection. In the clinical field, we found drug resistance genes in Staphylococcus epidermidis and Pseudomonas aeruginosa which were isolated from refractory UTI.These resistance genes were transferable to the other strains of causative bacteria of UTI.

我们研究了尿路感染（UTI）中的细菌和宿主因素。从尿路感染中分离出来的细菌，其表面有各种菌毛，可以粘附在尿道粘膜上。发现位于菌毛尖端的粘附素刺激多形核白细胞释放大量活性氧，对周围肾组织有毒，导致肾疤痕。预防肾疤痕形成是治疗肾脏感染最重要的事情之一。我们发现一些抗氧化剂如依布硒啉、乌司他丁和类固醇对肾脏疤痕有预防作用，这些物质可与抗菌药物联合使用治疗肾脏感染。在临床领域，我们在难治性尿路感染中分离到的表皮葡萄球菌和铜绿假单胞菌中发现了耐药基因，这些耐药基因可以转移到其他尿路感染致病菌中。

项目成果

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Haraoka M 等人：“Ebselen 对肾脏感染后大鼠肾脏疤痕的影响。”

Matsumoto T et al.: "Restorative effect of ofloxacin on the inhibited chemiluminescence response of neutrophils by sera from uremic patients" Drugs. 49. 75-77 (1995)

Matsumoto T 等人：“氧氟沙星对尿毒症患者血清抑制的中性粒细胞化学发光反应的恢复作用”药物。

Kubo S et al: "Inhibition of chemiluminescence response of human polymorphonuclear leukocytes by three different stinulation in hyperosmotic condition........" Renal Failure. 18. 69-74 (1996)

Kubo S 等人：“高渗条件下三种不同的刺激对人多形核白细胞化学发光反应的抑制......”肾功能衰竭。

Haraoka M et al.: "Effect of prednisolone on ascending renal infection due to biofilm disease and lower urinary fract obstruction in rats." Urological Research. 22. 383-387 (1995)

Haraoka M 等人：“泼尼松龙对大鼠生物膜病和下尿路梗阻引起的上行肾感染的影响。”

Takahashi K et al: "Direct inactivation of superaride generation of pmals by hyperosmoticurea" Journal of Urology. 149. 386-389 (1993)

Takahashi K 等人：“高渗尿素直接灭活 pmals 的 superaride 生成”，《泌尿学杂志》。