Study for the physiological functions of cytoskeleton-related calmodulin-binding proteins.

Ca^<2+>是细胞内最重要的信使之一，参与许多细胞功能，包括收缩、内吞、胞吐和细胞分裂。钙<2+>信号是由钙<2+>受体蛋白如肌钙蛋白C和钙调蛋白介导的。细胞骨架是由蛋白质丝组成的三维细胞内网状结构，这些蛋白质丝是细胞功能的分子基础。我们一直在研究这些功能的Ca^<2+>依赖性调节，并发现了一组钙调素结合蛋白也与细胞骨架相互作用。在本研究中，我们研究了钙调素结合蛋白介导的Ca^<2+>-钙调素依赖的细胞骨架调控机制：1。Ca^<2+>和钙调蛋白对map -actin相互作用的调控与Con A受体盖顶相关的caldesmon在脾t淋巴细胞上的重分布。3 . TPA诱导3T3细胞中肌动蛋白和钙蛋白的重组。Ca^<2+>-依赖性调节…更多膜下细胞骨架。蛋白4.1-钙调蛋白复合物对谱蛋白-肌动蛋白相互作用的调控。细胞胞嘧啶（一种35 kDa的细胞骨架相互作用和钙调素结合蛋白）的功能。一种新的84/82 kDa钙调素结合蛋白突触蛋白的鉴定。8 .牛肾上腺髓质Ca^<2+>-依赖性肌动蛋白的纯化。由caldesmon聚集体引起的f -肌动蛋白交联，而不是由其二聚体引起的。肌凝蛋白轻链多重磷酸化与肌凝蛋白atp酶活性的关系caldesmon对原肌球蛋白增强肌球蛋白atp酶活性的调控。肾上腺髓质肌动蛋白中肌动蛋白-肌球蛋白相互作用的双重调控。14.动脉平滑肌caldesmon和肌球蛋白轻链激酶的表征。-caldesmon 35 kDa功能片段的分离。原肌球蛋白在肌动蛋白-丝蛋白相互作用中的作用。caldesmon对三维收缩的调节。平滑肌细胞表型改变过程中-和-caldesmons的表达。少

Ca^<2+> is one of the most important intracelluar messenger in many cellular functions including contraction, endocytosis, exocytosis and cell division. Ca^<2+> signals are mediated by Ca^<2+>-receptive proteins such as troponin C and calmodulin. Cytoskeleton is the three-dimensional intracelluar meshwork of protein filaments which are molecular bases of these celluar functions. We have been studing the Ca^<2+>-dependent regulation of these functions, and have discovered a group of calmodulin-bindig proteins which also interact with cytoskeleton. In this study, we have investigated the Ca^<2+>-calmodulin dependent regulatory mechanism of cytoskeleton mediated by the cytoskeleton-interacting calmodulin-binding proteins:1. Regulation of MAPs-actin interaction by Ca^<2+> and calmodulin.2. Redistribution of caldesmon associated with Con A receptor capping on splenic T-lymphocytes.3. Reorganization of actin and calspectin in 3T3 cells induced by TPA treatment.4. Ca^<2+>-dependent regulation … More of subemembranous cytoskeleton.5. Regulation of spectrin-actin interaction by protein 4.1-calmodulin complex.6. Function of cytosynalin, a 35 kDa cytoskeleton-interacting and calmodulin-binding protein.7. Identification of a new 84/82 kDa calmodulin-binding protein as synapsin I.8. Purification of an 80 kDa Ca^<2+>-dependent actin-severing protein from bovein adrenal medulla.9. Crosslinking of F-actin caused by caldesmon aggregates, not by its dimers.10. Relationship of the multiple phosphorylation of myosin light chain and myosin ATPase activity.11. Regulation of the tropomyosin-enhanced myosin ATPase activity by caldesmon.12. Dual regulation of the actin-myosin interaction in adrenal medullary actomyosin.13. Characterization of caldesmon and myosin light chain kinase from arterial smooth muscle.14. Isolation of the 35 kDa functional fragment of -caldesmon.15. Role of tropomyosin in the actin-filamin interaction.16. Regulation of the tree-dimentional contraction by caldesmon.17. Expression of - and -caldesmons during phenotype chage of smooth muscle cells. Less