The Functional Significance of Sporopollenin in the Morphogenesis of the Cellular Slime Moulds

孢粉质在细胞粘菌形态发生中的功能意义

• 批准号: 60540442
• 负责人: MAEDA Yasuo
• 金额: $ 1.15万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60540442/
• 关键词: cellular slime mould; Dictyostelium; sporopollenin; cellulose; stalk sheath; cell wall; complex glucan; グルコース

During the morphogenetic events of culmination of the cellular slime moulds, the biosynthesis and deposition of stalk sheath and cell wall are of particular for an understanding of the mechanism of morphogenesis as well as the establishment of cell shape and rigidity. An alkali- and acid-resistant polymer (sporopollenin) is located on the stalk sheath of fruiting bodies as a bundle of fine fibrils (ca. 4 nm thick). Both the stalk sheath-constituting polymer (referred as to SSM) and the intact stalk cell wall were shown by several kinds of chemical analysis to be a homo-polymer of glucose. The stalk cell wall seemed to be composed of cellulose, as described previously. However, it is unlikely that the SSM is cellulose, because it is quite resistant to extensive cellulase-treatment and also is negative to PAS (periodic acid Schiff) reaction. Taken together these results, it is supposed that the SSM is a tight network of homo-polymer of glucose with a great many of branchings, thus being highly resistant to various chemical treatments. The biological significance of SSM is discussed, with special reference to the late developmental stages of slime mould cells.

在细胞型黏菌的形态发生过程中，茎鞘和细胞壁的合成和沉积对于了解形态发生的机制以及细胞形状和刚性的建立具有重要意义。一种耐碱性和耐酸性的聚合物（孢粉素）位于子实体的茎鞘上，作为一束细纤维（约100毫米）。4nm厚）。几种化学分析表明，构成茎鞘的聚合物（简称SSM）和完整的茎细胞壁都是葡萄糖的均聚物。如前所述，茎细胞壁似乎由纤维素组成。然而，SSM不太可能是纤维素，因为它对广泛的纤维素酶处理非常耐受，并且对PAS（高碘酸希夫）反应也是阴性的。综合这些结果，推测SSM是具有大量分支的葡萄糖均聚物的紧密网络，因此对各种化学处理具有高度抗性。SSM的生物学意义进行了讨论，特别是关于黏菌细胞的后期发育阶段。

项目成果

Y.Maeda: Journal of General Microbiology. 131. 201-205 (1985)

Y.Maeda：普通微生物学杂志。

T.Abe & Y.Maeda: Journal of General Microbiology. 132. 2787-2791 (1986)

阿部

前田靖男: 電子顕微鏡. 19. 165-171 (1985)

前田康夫：电子显微镜 19. 165-171 (1985)

Y. Maeda: "A new method for inducing synchronous growth of Dictyostelium discoideum cells using temperature shifts" Journal of General Microbiology. 132. 1189-1196 (1986)

Y. Maeda：“一种利用温度变化诱导盘基网柄菌细胞同步生长的新方法”普通微生物学杂志。

Y.Maeda: Journal Cell Science. 66. 297-308 (1984)

Y.Maeda：细胞科学杂志。