Localizations of neuropeptides in the brain and regulation of pituitary hormone secretion.

1. 胆囊收缩素（CCK）和P物质（SP）对垂体激素释放的影响。将含有CCK胆固醇混合物（重量比1:1或5:3）和含有SP和胆固醇混合物（重量比1:1）的camcamer植入雄性大鼠的中突。CCK可降低血清促黄体生成素（LH）浓度，但对促卵泡激素、催乳素和促甲状腺激素（TSH）浓度无显著影响；SP仅降低血清TSH浓度。植入腹内侧核或弓形核的CCK对血清LH浓度没有影响。由此可见，CCK抑制LH的释放，而SP抑制TSH的释放，可能是通过抑制LH释放激素和TSH释放激素从它们各自的轴突末端在正中凸起处的分泌。文昌鱼中枢神经系统神经肽的免疫组织化学研究。检测到核周和/或纤维对met -脑啡肽、b-内啡肽、精氨酸抗利尿素、催产素、血管紧张素II和鲤鱼尿紧张素I抗血清有免疫反应的神经元。发现在局部的孢子中密集地积聚了脑啡肽免疫反应物质。在积累的物质附近经常可见小的组织间隙，表明该物质可能释放并具有内分泌性质。由此推测精氨酸抗利尿激素免疫反应纤维与罗德细胞有轴突和轴体接触。2 .中枢神经系统对心房利尿多肽、促肾上腺皮质激素释放激素和亮氨酸-脑啡肽抗血清均无免疫反应。鱼脑内的房利钠多肽。应用hANP抗血清，采用PAP法研究ANP的免疫组化定位。虹鳟视顶膜室管膜层可见免疫阳性神经元。它们向视顶盖表面延伸轴突。

1. Effects of cholecystokinin(CCK) and substance P(SP) on pituitary hormone Release. A cammula containing a mixture of CCK cholesterol(1:1 or 5:3 by weight) and one containing a mexture of SP and cholesterol(1:1) were implanted into the mediam eminence of male rats. CCK reduced serum luteinizing hormone(LH) but not follicle stimulating hormone, prolactin and thyroid stimulating hormone(TSH) concentrations; SP reduced only serum TSH concentrations. CCK implanted into the ventromedial or arcuate nuclues failed to have any effect on serum LH concentration. It is thus concluded that CCK inhibits the release of LH, and SP inhibits the release of TSH, probably by inhibiting LH-releasing hormone and TSH-releaseing hormone sectetion from their respective axon terminals in the median eminence.2. An immunohistochemical study of neuropeptides in the central nervous system of amphioxus. Neurons with perikarys and/or fibers showing immunoreactions to antisera against Met-enkephalin, b-endorphin, arginine vasopressin, oxytocin, angiotensin II and carp urotensin I were detected. Met-enkiphalin immunoreactive material was found to accumulate densely in the localized spors. small tissue spaces were often seen adjacent to the accumulated material, suggesting possible release and an endoctine nature of this substance. It was surmized that the arginine vasopressin-immunoreactive fibers had axo-axonic and axo-somatic contacts with the Rohde cell. No immunoreactions to antisera against atrial netriuretic polypeptide, corticotropin-releasing hormone and Leu-enkephalin were detected in the central nervous system.3. Atrial natriuretic polypeptide (ANp) in the fish brain. The immunohistochemical localization of ANP was studied by the PAP method using hANP antiserum. immunopositive neurons were found in the ependymal layer of the optic tectum in the rainbow trout. They extended axons toward the surface of the optic tectum.