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Enzyme Electrode for Hydrogen Peroxide by the Use of ISFET and Its Analytical Application

ISFET过氧化氢酶电极及其分析应用

基本信息

批准号：
60550540
负责人：
WASA Tamotsu
金额：
$ 1.28万
依托单位：
University of Osaka Prefecture, College of Engineering
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

Developments of oxidation/reduction FET electrode(Redox-FET) and enzyme FET electrode which combined specificities of Redox-FET and enzyme were carried out in order to amplify the applicability of chemical sensor using FET (field effect transistor) and the following results were obtained:1. Measurement apparatus for single and triple electrodes with conventional and operational amplifier were constructed.2. FET electrodes by vacuum evaporation of various metals(Au, Pt, Au-Cr, Pt-Cr, etc.) on the gate of ISFET(ion-sensitive field effect transistor) were made. FET electrode by sputtering Pt had enough mechanical strength and Nernstian response(58-60 mV/decade) to hexacyanoferrate ( <II> / <III> ) system.3. Hydrogen peroxide (FET) electrode immobilized peroxidase(POD) was made. It has been ovserved that this electrode shows Nernstian response to 0.002-1 mM hydrogen peroxide in the buffer solution containing over 50 mM hexacyanoferrate( <II> ).4. Glucose (FET) electrode co-immobilized POD and glucose oxidase(GOD) was made and showed Nernstian response(58-60 mV/decade) to 0.02-1 mM glucose in the buffer solution(pH 9.0) containing 10-50 mM hexacyanoferrate( <II> ).5. We proposed simple numerical model for enzyme FET electrode and examined its propriety. Furthermore some consideration was under taken on its response mechanism.6. For the actual analysis of samples, standard addition method, calibration method and Gran's plot method were examined. Analytical values of control serum obtained by Gran's plot method showed to agree with certificated value.7. Hereafter, we will make micro-FET based on oxidation-reduction potential in cooperation with specialized manufacturer and study its analytical application.

为了扩大场效应晶体管（FET）化学传感器的适用性，将氧化还原FET电极（Redox-FET）和酶效应FET电极结合起来，进行了氧化还原FET电极（Redox-FET）和酶效应FET电极的开发。构建了单电极和三电极测量装置，分别采用常规放大器和运算放大器。在ISFET（离子敏感场效应晶体管）栅极上真空蒸发各种金属（Au、Pt、Au- cr、Pt- cr等），制备了FET电极。溅射Pt的FET电极具有足够的机械强度和对六氰高铁（<II> / <III>）体系的Nernstian响应（58 ~ 60 mV/decade）。制备了过氧化氢（FET）电极固定过氧化物酶（POD）。已经观察到，该电极在含有超过50 mM六氰高铁酸盐（<II>）的缓冲溶液中对0.002-1 mM过氧化氢表现出Nernstian响应。制备了葡萄糖（FET）电极共固定化POD和葡萄糖氧化酶（GOD），在含有10-50 mM六氰铁酸盐（<II>）的缓冲溶液（pH 9.0）中，对0.02-1 mM葡萄糖表现出58-60 mV/ 10年的Nernstian反应。提出了酶场效应管电极的简单数值模型，并对其适用性进行了检验。此外，还对其反应机制进行了一些审议。针对样品的实际分析，考察了标准添加法、标定法和Gran图法。用格兰图法测定的对照血清分析值与鉴定值一致。今后，我们将与专业厂家合作研制基于氧化还原电位的微型场效应管，并研究其分析应用。