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Transient intracellular calcium movement coupled with mechanical activity of smooth muscle.

短暂的细胞内钙运动与平滑肌的机械活动相结合。

基本信息

批准号：
60570040
负责人：
HOTTA Ken
金额：
$ 1.09万
依托单位：
Nagoya City University Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570040/
关键词：
Smooth muscle Single cell Ca transient Ca store Quin2 フィラ2

项目摘要

We have shown previously(J.J.P.35:1079,1985) that, intracellular free Ca in smooth muscle cells(guina pig jejunum) increased transiently upon stimulation followed by the mechanical response. We explored this study to the single cell using Fura 2.The isolated cell was obtained from taenia coli of guiea pig according to the method described by Momose & Gomi in 1979(J.Pharmaco dynamics 1:184). Fura2 was loaded into the cell by incubating the sample in HEPES buffer containing Fura 2AM for 30 min at 37゜C. The photomultiplier was set to recieve the emission(470nm) light from only one cell on the stage of microscope.Upon application of electric pulse, intensity of fluorecence light from the muscle begun to increase immedately after application of stimulus reaching to the maximum within one sec and returned to the original level after 2 sec. Concentration of intracellular freeCa was estimated as approximately <10^(-7)> M and the order of <10^(-6)> M,at rest and the maximum,respectively. The rate of light intensity change was faster than that observed previously in the tissue preparation. The speed of contraction following the stimulation was much slower than that of intracellular Ca transient. The results obtained here,together with previous one,suggest that intracellulary stored Ca may play certain role for the mechanical activation of the smooth muscle cell.

我们以前已经表明（J.J.P.35：1079，1985），平滑肌细胞（豚鼠空肠）中的细胞内游离Ca在刺激后瞬时增加，随后是机械反应。本研究采用Fura 2单细胞培养技术，从豚鼠结肠带绦虫中分离细胞，按Momose & Gomi（1979）的方法（J.Pharmaco dynamics 1：184）进行。通过将样品在含有Fura 2AM的HEPES缓冲液中在37 ℃下孵育30分钟将Fura 2加载到细胞中。将光电倍增管设置为只接收显微镜载物台上一个细胞发出的470 nm光，施加电脉冲后，肌肉发出的荧光强度立即开始增加，在1秒内达到最大值，2秒后恢复到原来的水平。细胞内游离钙浓度估计分别为<10^（-7）> M和<10^（-6）> M。光强度变化的速率比先前在组织制备中观察到的更快。刺激后收缩的速度远低于胞内钙瞬变的速度。本实验结果与前人的实验结果相结合，提示细胞内钙在平滑肌细胞的机械激活中可能起一定的作用。