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Structure and the cellular location of the novel phosphonoglycosphingolipids in the nervous tissues of Aplysia

海兔神经组织中新型磷酸鞘糖脂的结构和细胞定位

基本信息

批准号：
60570108
负责人：
ARAKI Shigeko
金额：
$ 0.96万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

On two-dimensional TLC, each tissue of Aplysia showed a pattern of phosphonoglycosphingolipids characteristic to the tissue. FGL-IIa and FGL-IIb were specific to the nerve fiber and FGL-V (G-13) was seemed to be specifically concentrated in the nervous tissues. Three novel phosphonoglycosphingolipids (FLG-IIa, FGL-IIb and FGL-V) were isolated from the nervous tissues of Aplysia kurodai, using three systems of silicic acid column chromatography and characterized. FGL-IIa was monophosphonoglycosphingolipid having an oligosaccharide chain consisting of glucose, galactose and galactosamine. FGL-IIb was also monophosphonoglycosphingolipid having fucose, glucose, galactose and galactosamine as an oligosaccharide moiety. FGL-V (G-13) seemed to be diphosphonoglycosphingolipid having an oligosaccharide chain consisting of glucose, galactose, galactosamine and 4-0-methylglucosamine.Antisera against FGL-IIa and FGL-IIb mixture were raised in rabbits. Two-dimensional thin-layer chromatography combined with immunoautoradiography using the antisera and <^(125)I> -Ig,F(ab')2, revealed nine immunoreactive glycolipids in extracts from the nerve fiber. By immunohistochemistry with the antisera and HRP-labelled antirabbit IgG, distinct and strong staining was observed in the nerovus tissue. Nerve fibers in the skin and muscle tissue were also stained.

在二维薄层色谱上，海兔的每个组织都显示出该组织特有的磷酸鞘糖脂模式。 FGL-IIa和FGL-IIb是神经纤维特异性的，而FGL-V (G-13)似乎专门集中在神经组织中。使用三种硅酸柱色谱系统从黑海兔的神经组织中分离出三种新型磷酸鞘糖脂（FLG-IIa、FGL-IIb 和 FGL-V）并进行表征。 FGL-IIa是具有由葡萄糖、半乳糖和半乳糖胺组成的寡糖链的单膦酰基鞘糖脂。 FGL-IIb也是具有岩藻糖、葡萄糖、半乳糖和半乳糖胺作为寡糖部分的单膦酰基鞘糖脂。 FGL-V(G-13)似乎是具有由葡萄糖、半乳糖、半乳糖胺和4-0-甲基葡萄糖胺组成的寡糖链的二膦酰基鞘糖脂。在兔中制备针对FGL-IIa和FGL-IIb混合物的抗血清。二维薄层色谱法与使用抗血清和<^(125)I>-Ig,F(ab')2的免疫放射自显影相结合，揭示了神经纤维提取物中的九种免疫反应性糖脂。通过使用抗血清和 HRP 标记的抗兔 IgG 进行免疫组织化学分析，在神经组织中观察到明显且强烈的染色。皮肤和肌肉组织中的神经纤维也被染色。