Research of egg specific glycosphingolipids-binding protein

我们从黑代黑桃卵中分离出一种新的糖脂EGL-II，并鉴定出其结构如下。(1)氨基酸分析表明，2-AEPn和葡萄糖胺的摩尔比为3:1。(2)神经酰胺的主要脂肪族成分为棕榈酸、硬脂酸和抗异索膦酸-4-括约碱。(3) EGL-II由葡萄糖/半乳糖/ n -乙酰氨基葡萄糖/3- o -甲基半乳糖按1:5:1的摩尔比组成，2- aepn分别附着于2 mol半乳糖和1 mol葡萄糖上。(4)根据过甲基化研究结果以及FAB-MS和SIMS光谱的片段图，得出EGL-II的糖序列和连锁为。<<化学式>>Gal1-3(GlcNAc1-2)Gal1-3(3-O-MeGal1-2)Gal1-3(Gal1-2) Gal1-3(Gal1-2)Gal1-4Glc1—Cer(5)质子磁共振数据显示，在球端区有β-Glc、β-Gal、α-GlcNAc、α-3-O-MeGal和4个α-Gal质子。

We isolated a novel glycolipid, EGL-II, in the egg of Aplysia kurodai and identified its structure as follows.(1) Amino acid analysis showed the presence of 2-AEPn and glucosamine in a molar ratio of 3 : 1.(2) The major aliphatic components of ceramide were palmitic acid, stearic acid and anteisononadeca-4-sphingenine.(3) EGL-II was composed of glucose/galactose/N-acetylglucosamine/3-O-methylgalactose in a molar ratio of 1 : 5 : 1 : 1 and 2-AEPn was attached to each of 2 mol of galactose and 1 mol of glucose.(4) From the results of the permethylation studies and the fragmentation diagram of the FAB-MS and SIMS spectra, the sugar sequence and linkage of EGL-II was concluded to be.<<chemical formula>>Gal1-3(GlcNAc1-2)Gal1-3(3-O-MeGal1-2)Gal1-3(Gal1-2)Gal1-4Glc1--Cer(5) The proton magnetic resonance data revealed β-Glc, β-Gal, α-GlcNAc, α-3-O-MeGal, and four α-Gal protons in the anomeric region.