Study on urinary mutagen in smokers

对61名吸烟者和107名不吸烟者的健康成年男性尿液进行了沙门氏菌/微粒体致突变性试验。在定量检测中，二氯甲烷适合于从XAD-2树脂柱上洗脱吸烟者尿液的致突变性。我们检测了这些二氯甲烷洗脱物的凝集物的致突变性，发现吸烟者的尿液的致突变性明显高于不吸烟者的尿液。在吸烟者组中，“尿液致突变性”与“收集尿液当天实际吸烟量”或“平均每天吸烟量”之间的相关系数具有统计学意义。吸烟者尿液中的这种致突变活性在硅胶(Wako Gel S-1)上洗脱时，以甲醇组分洗脱。

The mutagenicity of urine from a healthy population of young adult men, including 61 smokers and 107 nonsmokers, was investigated with the Salmonella/microsome test using the tester strain S. typhymurium TA98. In quantifying the assay, the use of dichrolomethane was suitable for eluting the mutagenicity of smokers' urine from XAD-2 resin column. We assayed mutagenicity in condensates of such dichrolomethane eluates and found that the mutagenicity of smokers' urine was significantly higher than that of nonsmokers' urine. In the smokers' group, correlation coefficient between "the mutgenicity of the urine" and "the acutual number of cigarettes smoked on the day of urine collection" or "the average number of cigaretts smoked per day" was statistically significant.Such mutagenic activity in smokers' urine was eluted in methanol fraction, when the urine condensates was chromatographed on silica gel(Wako gel S-1).