Study on urinary mutagen in smokers

吸烟者尿诱变剂的研究

• 批准号: 60570242
• 负责人: ISHINISHI Noburu
• 金额: $ 1.09万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570242/
• 关键词: Mutagenicity of urine; Ames test; Smoker; シリカゲルカラムクロマトグラフィー

The mutagenicity of urine from a healthy population of young adult men, including 61 smokers and 107 nonsmokers, was investigated with the Salmonella/microsome test using the tester strain S. typhymurium TA98. In quantifying the assay, the use of dichrolomethane was suitable for eluting the mutagenicity of smokers' urine from XAD-2 resin column. We assayed mutagenicity in condensates of such dichrolomethane eluates and found that the mutagenicity of smokers' urine was significantly higher than that of nonsmokers' urine. In the smokers' group, correlation coefficient between "the mutgenicity of the urine" and "the acutual number of cigarettes smoked on the day of urine collection" or "the average number of cigaretts smoked per day" was statistically significant.Such mutagenic activity in smokers' urine was eluted in methanol fraction, when the urine condensates was chromatographed on silica gel(Wako gel S-1).

使用测试菌株S通过沙门氏菌/微粒体试验研究了健康年轻成年男性人群（包括61名吸烟者和107名非吸烟者）尿液的致突变性。Typhymurium TA98.在定量试验中，用氯仿从XAD-2树脂柱上洗脱吸烟者尿液的致突变性是合适的。我们检测了这些三氯甲烷洗脱液冷凝物的致突变性，发现吸烟者尿液的致突变性显著高于非吸烟者尿液。吸烟组尿致突变性与尿收集日实际吸烟烟支数或平均每天吸烟烟支数的相关系数有统计学意义，尿浓缩物经硅胶（和子gel S-1）层析后，用甲醇洗脱尿致突变活性。

项目成果

Hiromi Kawano;Takeo Inamasu;Minoru Ishizawa;Noburu Ishinishi;Joichi Kumazawa: International and Archives occupational and Enviromental Health. 59. 1-9 (1987)

Hiromi Kawano；Takeo Inamasu；Minoru Ishizawa；Noburu Ishinishi；Joichi Kumazawa：国际与档案职业与环境健康。

河野博巳,稲益建夫,石沢実,北森成治,石西伸: 産業医学. 27. 166-167 (1985)

Hiromi Kono、Takeo Inamasu、Minoru Ishizawa、Seiji Kitamori、Shin Ishinishi：工业医学。 27. 166-167 (1985)

Hiromi Kawano, Takeo Inamasu, Minoru Ishizawa, Shigeji Kitamori and Noburu Ishinishi: "Analysis of mutagenicity in urine of young male smokers and nonsmokers." Jpn. J. Ind. Health. 27. 166-167 (1985)

Hiromi Kawano、Takeo Inamasu、Minoru Ishizawa、Shigeji Kitamori 和 Noburu Ishinishi：“年轻男性吸烟者和非吸烟者尿液中的致突变性分析。”

河野博巳,稲益建夫,北森成治,石西伸: 第57回日本産業衛生学会講演集. 372-373 (1984)

Hiromi Kono、Takeo Inamasu、Seiji Kitamori、Shin Ishinishi：第 57 届日本工业卫生学会会议记录 372-373 (1984)。

Hiromi Kawano, Takeo Inamasu, Minoru Ishixawa, Noburu Ishinishi and Joichi Kumazawa: "Mutagenicity of urine from young male smokers and nonsmokers." International Archives of Occupational and Environmental Health. 59. 1-9 (1987)

Hiromi Kawano、Takeo Inamasu、Minoru Ishixawa、Noburu Ishinishi 和 Joichi Kumazawa：“年轻男性吸烟者和非吸烟者尿液的致突变性。”