IMMUNO TOXICITY OF INORGANIC METAL COMPOUNDS

无机金属化合物的免疫毒性

• 批准号: 63480175
• 负责人: ISHINISHI Noburu
• 金额: $ 4.29万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480175/
• 关键词: NICKEL; MANGANESE; ARSENIC SELENIDE; CARCINOGENICITY; IMMUNO TOXICITY; ANIMAL EXPERIMENT; 砒化セレン; 無機金属化合物; 三酸化ヒ素; リンパ球芽球化反応; サイクロフォスファミド

Immuno toxicity of inorganic metal compounds was studied in male Syrian golden hamsters given intermittent intratracheal instillations.In first experiment, the tumorigenicity or interaction effect of metal nickel(Ni), as a positive control, metal manganese(Mn) and arsenic selenide(As2Se3) was studied in male Syrian golden hamsters which received each metal compounds containing a total dose of 7.5 mg Ni, Mn or As by means of intratracheal instillations once a week for 15 weeks. As a controls, some hamsters were treated with only the vehicle, phosphate buffer solution. All hamsters were observed during their entire life span. The materials treated by intratracheal instillations did not significantly effect the cumulative body-weight gain of the hamsters. The lower survival rate was observed in the Ni group compared with that of the Ni+Mn group, and the difference in the survival rates between Ni and Ni+Mn group was statistically significant. During the animal's total life span, one lung … More adenoma was seen in the 23 hamsters in the control group. But no tumors were observed in the respiratory tracts in any group except the control group. Besides lung tumor formation, columnar or cuboidal epithelial hyperplasia with or without squamous metaplasia or deposition of metal particles in the alveolar space or alveolar septa were observed in the Ni, Ni+Mn and As_2Se_3 group and the incidence rates of these lesions were significantly different from that in the control group. The total tumor incidence rates including the respiratory tracts were 8.7% in the control group, 4.0% in the Ni group, 26.9% in the Ni+Mn group and 10.3% in the As_2Se_3. The incidences of total tumors in the groups given Ni, Ni+Mn and As_2Se_3 were not significantly greater than that in the control group according the chi-square test. In the scanning electron microscopic findings, the main lesions observed in the alveolar region were the deposition of each metal particles, the accumulations of macrophages in the alveoli and the thickening the alveolar walls, which lesions were more frequently observed in the alveolar region than in the bronchiolar region.In second experiment, comparative chronic toxicity, including tumorigenicity, of arsenic trioxide(As_2O_3), gallium arsenide(GaAs), chromium trioxide(Cr_2O_3), nickel oxide(NiO) and benzo(a)pyrene(B(a)P), as a positive control, were studied using male Syrian golden hamsters given intermittent intratracheal instillations. GaAs particles were likely to produce relatively severe lung damage and the survival of the hamsters was shortened significantly compared with a control group. The total tumor incidence including the respiratory tracts was 3.3 % in the As_2o_3 group, 3.3% in the GaAs group, 0% in the Cr_2O_3, 3.3% in the NiO group, 6.8 % in the B(a)P group and 3.3% in the control group, and no significant difference between the each metal or B(a)P treatedgroup and the control group was found.From these study, the tumorigenicity of inorganic metal compounds were not ascertained in the respiratory tracts or other organs of hamsters. But it seems that Mn particles enhance the shortened survival period induced by Ni treated. Less

本实验研究了无机金属化合物对雄性叙利亚金黄地鼠的免疫毒性，第一个实验研究了金属镍（Ni）、金属锰（Mn）和硒化砷（As_2Se_3）作为阳性对照对雄性叙利亚金黄地鼠的致瘤性或相互作用，每种金属化合物总剂量为7.5mgNi，每周1次，连续15周。作为对照，一些仓鼠仅用载体磷酸盐缓冲液处理。所有仓鼠在其整个寿命期间进行观察。经腹膜内滴注处理的材料对仓鼠的累积体重增加没有显著影响。Ni组的存活率低于Ni+Mn组，且Ni组与Ni+Mn组之间的存活率差异有统计学意义。在动物的整个生命周期中，一个肺 ...更多信息 在对照组的23只仓鼠中观察到腺瘤。但除对照组外，其他各组呼吸道均未见肿瘤。Ni、Ni+Mn和As_2Se_3组除有肺肿瘤形成外，还出现柱状或立方上皮增生伴或不伴鳞状上皮化生，肺泡腔或肺泡隔内有金属颗粒沉积，其发生率与对照组相比有显著性差异。含呼吸道肿瘤的总发生率，对照组为8.7%，Ni组为4.0%，Ni+Mn组为26.9%，As_2Se_3组为10.3%。经卡方检验，Ni组、Ni+Mn组和As_2Se_3组的总肿瘤发生率与对照组相比无显著性差异。在扫描电镜观察中，肺泡区的主要病变是各种金属颗粒的沉积、肺泡内巨噬细胞的聚集和肺泡壁增厚，其中肺泡区的病变多于细支气管区。用雄性叙利亚金黄地鼠作实验，以砷化镓（GaAs）、三氧化铬（Cr_2O_3）、氧化镍（NiO）和苯并（a）芘（B（a）P）为阳性对照。砷化镓颗粒可能会产生相对严重的肺损伤，与对照组相比，仓鼠的存活时间显着缩短。As_2O_3组、GaAs组、Cr_2O_3组、NiO组、B（a）P组和对照组的肿瘤发生率（包括呼吸道）分别为3.3%、3.3%、0%、3.3%、6.8%和3.3%，各金属或B（a）P处理组与对照组之间无显著性差异。无机金属化合物在仓鼠呼吸道或其他器官中的致瘤性未确定。但锰颗粒似乎增强了镍处理引起的存活期缩短。少

项目成果

HISANAGA, A.: "LUNG TUMORIGENICITY OF INORGANIC METAL COMPOUNDS." PROCEEDING OF WORKSHOP CONCERNING RARE METALS, THE JAPANESE SOCIETY FOR HYGIENE. 27-38 (1989)

Hisanaga, A.：“无机金属化合物的肺部致瘤性。”

久永 明: "人工新素材の慢性毒性および催腫瘍性について" 日本衛生学会ワークショップ「微量元素」報告論文集. 37-43 (1990)

Akira Hisanaga：“新型人造材料的慢性毒性和致瘤性”日本健康科学学会研讨会“微量元素”报告论文集37-43（1990）。

HISANAGA, A.: "CHRONIC TOXICITY AND TUMORIGENICITY OF SOME ADVANCED MATERIALS" PROCEEDING OF WORKSHOP CONCERNING RARE METALS, THE JAPANESE SOCIETY FOR HYGIENE. 37-44 (1990)

Hisanaga, A.：“某些先进材料的慢性毒性和致瘤性”日本卫生学会稀有金属研讨会论文集。

久永 明: "無機金属化合物による肺腫瘍性について" 日本衛生学会ワークショップ「微量元素」報告論文集. 27-38 (1989)

Akira Hisanaga：“无机金属化合物引起的肺致瘤性”日本健康科学学会研讨会“微量元素”报告论文集27-38（1989）。

久永 明: "人工新素材の慢性毒性および催腫瘍性について" 日本衛生学会ワ-クショップ「微量元素」報告論文集. 37-44 (1990)

Akira Hisanaga：“新型人造材料的慢性毒性和致瘤性”日本健康科学学会研讨会“微量元素”报告论文集37-44（1990）。