Evaluation of chemotherapeutic effect upon 203 GL glioma in mice as studied by p-31-NMR spectroscopy.

用P-31核磁共振波谱和流式细胞术评价化疗对小鼠脑胶质瘤的疗效。我们发现，ACNU或替加氟以低于LD-50的剂量给药导致在24或48小时后无机磷酸盐（Pi）/磷酸肌酸（PCr）和磷酸单酯（PME）/磷酸肌酸（PCr）的比率的部分抑制，尽管这些比率通常随着肿瘤的生长而增加。用流式细胞术分析胶质瘤细胞中G-2-M阻断后24-48小时内DNA四倍体的积累，但其变化时间稍晚于Pi/pcr比值的变化。另一方面，在给药后8天观察到组织学变化。因此，可以得出结论，在体内P-31-NMR光谱可以检测到肿瘤中的代谢途径的变化，早在24-48小时后，化疗药物给药。

The effect of chemotherapy against glioma in mouse was evaluated by P-31 NMR spectroscopy and flow cytometry. We found that administration of ACNUor tegafur at a dose less than LD-50 resulted in the partial suppression of the ratio of inorganic phosphate (Pi)/phosphocreatine (PCr) and phosphomonoester (PME)/creatine phosphate (PCr) after 24 or 48 hrs although these ratios are usually increased together with growth of tumors. Flow cytometric analysis of glioma in vivo showed an accumulation in cells containing tetraploid DNA by G-2-M block in 24-48 hrs after treatment However, the change occurred at a period slightly later than that of Pi/pcr ratio. On the other hand, histological change was noted at 8 days after administration. Hence, it is concluded that in vivo P-31-NMR spectroscopy can detect a change of metabolic pathways in tumors as early as 24-48 hr after administration of chemotherapeutic agents.