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Evaluation of chemotherapeutic effect upon 203 GL glioma in mice as studied by p-31-NMR spectroscopy.

通过 p-31-NMR 波谱研究评估小鼠 203 GL 神经胶质瘤的化疗效果。

基本信息

批准号：
60570474
负责人：
NISHIKAWA Junichi
金额：
$ 1.15万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570474/
关键词：
NMR P-31-NMR spectroscopy chemotherapy Glioma 【^(31)P】-NMR-スペクトロスコピー全身用MR装置

项目摘要

The effect of chemotherapy against glioma in mouse was evaluated by P-31 NMR spectroscopy and flow cytometry. We found that administration of ACNUor tegafur at a dose less than LD-50 resulted in the partial suppression of the ratio of inorganic phosphate (Pi)/phosphocreatine (PCr) and phosphomonoester (PME)/creatine phosphate (PCr) after 24 or 48 hrs although these ratios are usually increased together with growth of tumors. Flow cytometric analysis of glioma in vivo showed an accumulation in cells containing tetraploid DNA by G-2-M block in 24-48 hrs after treatment However, the change occurred at a period slightly later than that of Pi/pcr ratio. On the other hand, histological change was noted at 8 days after administration. Hence, it is concluded that in vivo P-31-NMR spectroscopy can detect a change of metabolic pathways in tumors as early as 24-48 hr after administration of chemotherapeutic agents.

用P-31核磁共振波谱和流式细胞仪评价化疗对小鼠脑胶质瘤的治疗效果。我们发现，在给药剂量低于LD-50的ACNU或替加氟24或48小时后，无机磷(PI)/磷酸肌酸(PCr)和磷酸单酯(PME)/磷酸肌酸(PCr)的比率部分受到抑制，尽管这些比率通常随着肿瘤的生长而增加。在体脑胶质瘤的流式细胞仪分析显示，在治疗后24-48小时，含有四倍体DNA的细胞被G-2-M阻断，但这种变化发生的时间略晚于PI/PCR比值的变化。另一方面，在给药后第8天观察到组织学改变。因此，在体内，P-31-核磁共振波谱可以最早在给药后24-48小时检测到肿瘤代谢途径的变化。